Vet World Vol.15 June-2022 Article-23
Research Article
Veterinary World, 15(6): 1583-1588
https://doi.org/10.14202/vetworld.2022.1583-1588
Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
2. Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University, Denpasar-Bali, Indonesia.
3. Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Background and Aim: Breast cancer is the most common type of cancer in women because it attacks the productive age. Preliminary studies showed that lactic acid bacteria (LAB) strain SR6 from the Bali cattle colon has the potential to act as a superior probiotic. It is also assumed that its bacteriocin structure is specific and has a strong relationship with the specificity of the ligand and its biological activity at a receptor. Therefore, this study aims to assess the use of local LAB strains, which produce bacteriocins as anticancer agents, as well as to identify the bacteria as potent producers molecularly.
Materials and Methods: The study was initiated by cultivating LAB SR6 strain from stock isolates on De Man, Rogosa, and Sharpe (Oxoid, CM 0369, England) broth media. It was then confirmed molecularly through analysis of the 16S ribosomal ribonucleic acid gene. Subsequently, its anticancer activity was tested by assessing the cytotoxic activity in T47D cell culture using the 3-(4, 5 dimetiltiazol-2-yl)-2.5-diphenyl tetrazolium bromide (Invitrogen M6494, US) method.
Results: The results showed that the LAB strain SR6 was identified molecularly as Pediococcus pentosaceus. Furthermore, it had a toxic effect on T47D cells, which was indicated by the number of deaths after treatment with the extracellular protein of the strain, especially at the 50% total cell volume level.
Conclusion: Based on the toxic effect of the strain on human T47D cells, the LAB SR6 isolate, which was identified as P. pentosaceus has the potential to be developed as a good anticancer drug against breast cancer. However, there is a need to carry out an integrated study to fully explore the suitability of bacteriocins as in vivo therapeutics against the disease completely. Keywords: apoptosis, breast cancer, lactic acid bacteria SR6 strain, necrosis.
Keywords: apoptosis, breast cancer, lactic acid bacteria SR6 strain, necrosis.
How to cite this article: Swacita IBN, Suardana IW, Sudisma IGN, Wihadmadyatami H (2022) Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line, Veterinary World, 15(6): 1583–1588.
Received: 25-02-2022 Accepted: 18-05-2022 Published online: 29-06-2022
Corresponding author: I. Wayan Suardana E-mail: wayan_suardana@unud.ac.id
DOI: 10.14202/vetworld.2022.1583-1588
Copyright: Swacita IBN, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.