Vet World Vol.15 March-2022 Article-17
Research Article
Veterinary World, 15(3): 672-678
https://doi.org/10.14202/vetworld.2022.672-678
Hematological indices and their correlation with glucose control parameters in a prediabetic rat model
2. Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
3. Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
4. Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Background and Aim: Chronic hyperglycemia in prediabetic individuals would progress to diabetes and lead to several systemic disruptions, including hematological parameters. This study aimed to investigate the correlation between prediabetes and hematological indices in a prediabetic rat model.
Materials and Methods: Eighteen male rats were randomly divided into two groups of nine. Prediabetes was induced in nine rats by a 3-week high-fat and high-glucose diet, followed by low-dose streptozotocin (STZ) injection (30 mg/ kg body weight). The oral glucose tolerance test was performed, and the fasting blood glucose (FBG) and insulin levels were measured 72 h after STZ administration. The control group of nine rats was given standard diets. At the end of the 3rd week, the animals fasted overnight before blood collection. Blood samples were drawn and used for the analysis of the FBG and fasting insulin levels and glycated albumin to define prediabetes criteria before hematology analysis.
Results: We found a significant increase in the FBG and insulin levels in the prediabetic versus the control group. There were decreases in red blood cells, hemoglobin, and hematocrit levels and red cell distribution in prediabetic rats versus the control. At the same time, a significant increase in the platelet count was observed in the prediabetic group. There was a positive correlation between FBG and lymphocytes and neutrophil-lymphocyte ratio in prediabetic rats. On the other hand, we found a negative correlation between white blood cell count and glycated albumin.
Conclusion: Correlations were found in several hematological parameters in the prediabetic rat models. The changes in hematological indices in prediabetic rats may be further used as a valuable indicator of glycemic control. Keywords: diabetes mellitus, high-fat and high-glucose diet, hyperglycemia, neutrophil-lymphocyte ratio, streptozotocin.
Keywords: diabetes mellitus, high-fat and high-glucose diet, hyperglycemia, neutrophil-lymphocyte ratio, streptozotocin.
How to cite this article: Krisnamurti DGB, Purwaningsih EH, Tarigan TJE, Soetikno V, Louisa M (2022) Hematological indices and their correlation with glucose control parameters in a prediabetic rat model, Veterinary World, 15(3): 672-678.
Received: 04-11-2021 Accepted: 04-02-2022 Published online: 24-03-2022
Corresponding author: Melva Louisa E-mail: melva.louisa@gmail.com
DOI: 10.14202/vetworld.2022.672-678
Copyright: Krisnamurti DGB, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.