Vet World Vol.15 March-2022 Article-32
Research Article
Veterinary World, 15(3): 782-788
https://doi.org/10.14202/vetworld.2022.782-788
Association of pleomorphic adenoma gene 1 with body weight and measurement of Bali cattle (Bos javanicus)
2. Research Center for Biotechnology, Research Organization for Life Sciences, National Research and Innovation Agency (BRIN), Cibinong, Jawa Barat, Indonesia.
3. Assessment Institute for Agricultural Technology – Central Java, Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Semarang, Indonesia.
Background and Aim: Pleomorphic adenoma gene 1 (PLAG1) encodes a multifunctional transcription factor that controls many genes and pathways and is associated with cattle body weight and measurements. This study aimed to evaluate the association between PLAG1 polymorphisms with body weight and measurements in Bali cattle.
Materials and Methods: A total of 87 Bali cattle, consisting of 48 bulls and 39 heifers at the Breeding Center for Bali Cattle, were used as the population in this study. Cattle were 2 years old and kept semi-intensively in the pasture. Phenotype data consisting of body weight, withers height, body length, chest girth, waist height, and chest depth were measured. Birth weight data were obtained from birth records, and weight gain, adjusted weaning weight, and yearling weight were calculated using formulas. Blood samples were taken from the jugular vein as much as 5 mL, and genomic DNA was isolated using the salting-out method. Polymerase chain reaction (PCR) was performed to amplify three target polymorphisms, namely, g.48308 C>T, g.32212 (19 bp indel), and g.45233 T>C. The presence of a 19 bp indel was determined by direct observation of the PCR product on a 2% agarose gel. Two other polymorphisms were detected by PCR-restriction fragment length polymorphism using the restriction endonuclease enzymes SacII and BclI. PLAG1 genotype and phenotype associations were analyzed using a general linear model.
Results: The results showed that two of the target polymorphisms in PLAG1 did not vary. The DD genotype indicated by 123 bp of PCR product was the only genotype identified for g.32212 19 bp indel, and TT genotype was the only genotype found for g.45233 T>C single-nucleotide polymorphism (SNP). Conversely, g.48308 C>T SNP was found to be polymorphic. In addition, the g.48308 C>T polymorphism of PLAG1 was significantly associated with body length of Bali cattle. Cattle with the CC genotype had a greater body length than the other two genotypes.
Conclusion: The g.48308 C>T SNP in PLAG1 was associated with Bali cattle body length characteristics. This finding could be used as a basis for selecting Bali cattle based on body length characteristics. Keywords: association study, Bali cattle, body length, growth trait, Pleomorphic adenoma gene 1.
Keywords: association study, Bali cattle, body length, growth trait, Pleomorphic adenoma gene 1.
How to cite this article: Cahyadi M, Sukaryo S, Dhiaurridho MI, Bramastya TA, Yanti Y, Riyanto J, Volkandari SD, Sudrajad P (2022) Association of pleomorphic adenoma gene 1 with body weight and measurement of Bali cattle (Bos javanicus), Veterinary World, 15(3): 782-788.
Received: 08-11-2021 Accepted: 22-02-2022 Published online: 30-03-2022
Corresponding author: Muhammad Cahyadi E-mail: mcahyadi@staff.uns.ac.id
DOI: 10.14202/vetworld.2022.782-788
Copyright: Cahyadi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.