Vet World Vol.15 May-2022 Article-24
Research Article
Veterinary World, 15(5): 1333-1340
https://doi.org/10.14202/vetworld.2022.1333-1340
Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach
2. Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Background and Aim: Specific tumor biomarkers are useful for the early diagnosis of cancer or can predict the recurrence of neoplastic disease in humans and animals. Lymphoma in dogs could be classified into B-, T-, and NK-cell origins. T-cell lymphoma has the worst prognosis with a shorter survival time and disease-free interval. This study aimed to identify the differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using a tandem mass tag (TMT)-based quantitative proteomics.
Materials and Methods: Serum samples were collected from 20 untreated canine lymphomas (14 B-cells and 6 T-cells) and four healthy control dogs. Sera peptides from each sample were processed for TMT 10-plex tagging and analyzed using liquid chromatography-mass spectrometry (MS). Differential proteome profiling was then compared between lymphoma and control.
Results: We discovered 20 elevated and 14 decreased serum proteins in the lymphoma group relative to the healthy group. Six candidate increased proteins in canine lymphomas were beta-actin cytoplasmic 1 (ACTB, p=0.04), haptoglobin (p=0.002), beta-2 microglobulin (β2M, p=0.007), beta-2 glycoprotein 1 (APOH, p=0.03), metalloproteinase inhibitor 1 (TIMP-1, p=0.03), and CD44 antigen (p=0.02). When compared between B- and T-cell lymphomas, B-cell phenotypes had upregulated immunoglobulin (Ig) heavy chain V region GOM (p=0.02), clusterin (p=0.01), apolipoprotein C1 (APOC1, p=0.05), and plasminogen (p=0.02).
Conclusion: These findings were investigated quantitative serum proteomes between B- and T-cell lymphomas using TMT-based MS. ACTB, β2M, APOH, TIMP-1, CD44 antigen, Ig heavy chain V region GOM, and APOC1 are novel candidate proteins and might serve as a lymphoma biomarker in dogs. However, evaluation with an increased sample size is needed to confirm their diagnostic and prognostic ability. Keywords: B-cell lymphoma, dog, tandem mass tag proteomics, T-cell lymphoma.
Keywords: B-cell lymphoma, dog, tandem mass tag proteomics, T-cell lymphoma.
How to cite this article: Fonghem P, Pisitkun T, Rattanapinyopituk K, Sirivisoot S, Rungsipipat A (2022) Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach, Veterinary World, 15(5): 1333-1340.
Received: 21-01-2022 Accepted: 18-04-2022 Published online: 26-05-2022
Corresponding author: Sirintra Sirivisoot and Anudep Rungsipipat E-mail: sirintra.s@chula.ac.th and anudep.r@chula.ac.th
DOI: 10.14202/vetworld.2022.1333-1340
Copyright: Fonghem, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.