Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach

Vet World Vol.15 May-2022 Article-24

Research Article

Veterinary World, 15(5): 1333-1340

https://doi.org/10.14202/vetworld.2022.1333-1340

Piyanoot Fonghem¹, Trairak Pisitkun², Kasem Rattanapinyopituk¹, Sirintra Sirivisoot¹, and Anudep Rungsipipat¹

1. Center of Excellence for Companion Animal Cancer, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
2. Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Background and Aim: Specific tumor biomarkers are useful for the early diagnosis of cancer or can predict the recurrence of neoplastic disease in humans and animals. Lymphoma in dogs could be classified into B-, T-, and NK-cell origins. T-cell lymphoma has the worst prognosis with a shorter survival time and disease-free interval. This study aimed to identify the differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using a tandem mass tag (TMT)-based quantitative proteomics.

Materials and Methods: Serum samples were collected from 20 untreated canine lymphomas (14 B-cells and 6 T-cells) and four healthy control dogs. Sera peptides from each sample were processed for TMT 10-plex tagging and analyzed using liquid chromatography-mass spectrometry (MS). Differential proteome profiling was then compared between lymphoma and control.

Results: We discovered 20 elevated and 14 decreased serum proteins in the lymphoma group relative to the healthy group. Six candidate increased proteins in canine lymphomas were beta-actin cytoplasmic 1 (ACTB, p=0.04), haptoglobin (p=0.002), beta-2 microglobulin (β2M, p=0.007), beta-2 glycoprotein 1 (APOH, p=0.03), metalloproteinase inhibitor 1 (TIMP-1, p=0.03), and CD44 antigen (p=0.02). When compared between B- and T-cell lymphomas, B-cell phenotypes had upregulated immunoglobulin (Ig) heavy chain V region GOM (p=0.02), clusterin (p=0.01), apolipoprotein C1 (APOC1, p=0.05), and plasminogen (p=0.02).

Conclusion: These findings were investigated quantitative serum proteomes between B- and T-cell lymphomas using TMT-based MS. ACTB, β2M, APOH, TIMP-1, CD44 antigen, Ig heavy chain V region GOM, and APOC1 are novel candidate proteins and might serve as a lymphoma biomarker in dogs. However, evaluation with an increased sample size is needed to confirm their diagnostic and prognostic ability. Keywords: B-cell lymphoma, dog, tandem mass tag proteomics, T-cell lymphoma.

Keywords: B-cell lymphoma, dog, tandem mass tag proteomics, T-cell lymphoma.

How to cite this article: Fonghem P, Pisitkun T, Rattanapinyopituk K, Sirivisoot S, Rungsipipat A (2022) Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach, Veterinary World, 15(5): 1333-1340.

Received: 21-01-2022 Accepted: 18-04-2022 Published online: 26-05-2022

Corresponding author: Sirintra Sirivisoot and Anudep Rungsipipat E-mail: sirintra.s@chula.ac.th and anudep.r@chula.ac.th

DOI: 10.14202/vetworld.2022.1333-1340

Copyright: Fonghem, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.