Research Article | 09 Nov 2022

Effect of supplementing L-cysteine and its group analogs on frozen semen quality of bulls: A meta-analysis

Sari Yanti Hayanti1, Mohammad Miftakhus Sholikin1,2, Anuraga Jayanegara3,4, Mokhamad Fakhrul Ulum5, Marchie Astrid da Costa1, Fitriawaty Fitriawaty1, Surya Surya1, Maureen Chrisye Hadiatry1, Santiananda Arta Asmarasari1, Eko Handiwirawan1, Yenny Nur Anggraeny1, Eni Siti Rohaeni1, Salfina Nurdin Ahmad1, Bustami Bustami1, Aryogi Aryogi1, Dicky Pamungkas1, and Yenni Yusriani1Show more
VETERINARY WORLD | pg no. 2517-2524 | Vol. 15, Issue 11 | DOI: 10.14202/vetworld.2022.2517-2524
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Abstract

          
Background and Aim: The quality of frozen bull sperm after thawing is influenced by the primary diluent and antioxidant. This meta-analysis was conducted to determine the effect of supplementing L-cysteine and its group analogs on the quality of frozen bull sperm.
Materials and Methods: A total of 22 articles obtained from Google Scholar and Scopus were integrated into metadata. The effects of adding L-cysteine and its analogs (e.g., cysteine HCl and N-acetyl-L-cysteine), both of which are known as L-cysteine, were evaluated in this meta-analysis. The following parameters were examined: Abnormality, acrosome damage, acrosomal integrity, DNA damage, DNA integrity, malondialdehyde (MDA) content, plasma membrane integrity, pregnancy rate, progressive motility, sperm viability, and total motility. Data were analyzed using the mixed model methodology, with L-cysteine dosage as a fixed effect and different studies as random effects.
Results: L-cysteine supplementation significantly increased the total motility (p < 0.05) and MDA content of semen, following a linear pattern. Progressive motility, acrosomal integrity, and plasma membrane integrity were significantly increased, showing a quadratic pattern (p < 0.05). Abnormality and acrosome damage were significantly decreased (p < 0.05), following a quadratic and linear pattern, respectively. Other parameters remained unaffected by L-cysteine supplementation. L-cysteine and cysteine HCl significantly inhibited (p = 0.001) acrosome damage in thawed frozen sperm compared with control sperm.
Conclusion: Supplementing L-cysteine and its analog groups are recommended for freezing bull semen as it generally improves sperm quality. Keywords: bull cattle, cryopreservation, L-cysteine, meta-analysis, sperm.
          
Keywords: bull cattle, cryopreservation, L-cysteine, meta-analysis, sperm.