Effect of supplementing L-cysteine and its group analogs on frozen semen quality of bulls: A meta-analysis

Vet World Vol.15 November-2022 Article-2

Research Article

Veterinary World, 15(11): 2517-2524

https://doi.org/10.14202/vetworld.2022.2517-2524

Sari Yanti Hayanti¹, Mohammad Miftakhus Sholikin^1,2, Anuraga Jayanegara^2,3, Mokhamad Fakhrul Ulum⁴, Marchie Astrid da Costa¹, Fitriawaty Fitriawaty¹, Surya Surya¹, Maureen Chrisye Hadiatry¹, Santiananda Arta Asmarasari¹, Eko Handiwirawan¹, Yenny Nur Anggraeny¹, Eni Siti Rohaeni¹, Salfina Nurdin Ahmad¹, Bustami Bustami¹, Aryogi Aryogi¹, Dicky Pamungkas¹, and Yenni Yusriani¹

1. Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of The Republic of Indonesia (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16911, Indonesia.
2. Animal Feed and Nutrition Modelling Research Group, Faculty of Animal Science, IPB University, Bogor 16680, West Java, Indonesia.
3. Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, West Java, Indonesia.
4. Division of Veterinary Reproduction, Obstetrics, and Gynaecology, Department of Veterinary Clinic, Reproduction, and Pathology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, West Java, Indonesia.

Background and Aim: The quality of frozen bull sperm after thawing is influenced by the primary diluent and antioxidant. This meta-analysis was conducted to determine the effect of supplementing L-cysteine and its group analogs on the quality of frozen bull sperm.

Materials and Methods: A total of 22 articles obtained from Google Scholar and Scopus were integrated into metadata. The effects of adding L-cysteine and its analogs (e.g., cysteine HCl and N-acetyl-L-cysteine), both of which are known as L-cysteine, were evaluated in this meta-analysis. The following parameters were examined: Abnormality, acrosome damage, acrosomal integrity, DNA damage, DNA integrity, malondialdehyde (MDA) content, plasma membrane integrity, pregnancy rate, progressive motility, sperm viability, and total motility. Data were analyzed using the mixed model methodology, with L-cysteine dosage as a fixed effect and different studies as random effects.

Results: L-cysteine supplementation significantly increased the total motility (p < 0.05) and MDA content of semen, following a linear pattern. Progressive motility, acrosomal integrity, and plasma membrane integrity were significantly increased, showing a quadratic pattern (p < 0.05). Abnormality and acrosome damage were significantly decreased (p < 0.05), following a quadratic and linear pattern, respectively. Other parameters remained unaffected by L-cysteine supplementation. L-cysteine and cysteine HCl significantly inhibited (p = 0.001) acrosome damage in thawed frozen sperm compared with control sperm.

Conclusion: Supplementing L-cysteine and its analog groups are recommended for freezing bull semen as it generally improves sperm quality. Keywords: bull cattle, cryopreservation, L-cysteine, meta-analysis, sperm.

Keywords: bull cattle, cryopreservation, L-cysteine, meta-analysis, sperm.

How to cite this article: Hayanti SY, Sholikin MM, Jayanegara A, Ulum MF, da Costa MA, Fitriawaty F, Surya S, Hadiatry MC, Asmarasari SA, Handiwirawan E, Anggraeny YN, Rohaeni ES, Ahmad SN, Bustami B, Aryogi A, Pamungkas D, and Yusriani Y (2022) Effect of supplementing L-cysteine and its group analogs on frozen semen quality of bulls: A meta-analysis, Veterinary World, 15(11): 2517–2524.

Received: 10-07-2022 Accepted: 19-09-2022 Published online: 09-11-2022

Corresponding author: Sari Yanti Hayanti E-mail: drh.sari.bptpjambi@gmail.com

DOI: 10.14202/vetworld.2022.2517-2524

Copyright: Hayanti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.