Vet World Vol.15 September-2022 Article-24
Research Article
Veterinary World, 15(9): 2309-2314
https://doi.org/10.14202/vetworld.2022.2309-2314
Molecular detection and dense granule antigen 6 genotyping of feline Toxoplasma gondii in Phayao, Thailand
2. Department of Applied Statistics, Rajamangala University of Technology Isan, Khon Kaen Campus, Khon Kaen, Thailand.
3. Scientific Instrument and Product Standard Quality Inspection Center, University of Phayao, Phayao, Thailand.
Background and Aim: Globally, toxoplasmosis is an important zoonotic parasite infection of many warm-blooded animals (including humans). Toxoplasma gondii oocysts are widespread, and their contamination can be primarily attributed to the members of the Felidae family. This study aimed to estimate the prevalence and determine the dense granule antigen 6 (GRA6) genotype of T. gondii among domestic cats in the Phayao Province, Thailand.
Materials and Methods: A total of 124 fecal samples were collected from owned cats in the Muang district, Phayao, Thailand, from January to December 2020. Fecal samples were tested for the presence of T. gondii DNA using targeted B1 gene polymerase chain reaction (PCR) amplification, and positive samples were subsequently analyzed for their T. gondii genotype through PCR-restriction fragment length polymorphism (RFLP) analysis and sequencing of the GRA6 gene.
Results: Among the 124 samples, 46 (37.1%) were tested positive for T. gondii. Only 10 positive DNA samples were successfully amplified for the GRA6 marker. Subsequent PCR-RFLP and sequence analyses indicated that all T. gondii isolates from cats in Phayao belonged to GRA6 genotype I.
Conclusion: Data revealed that toxoplasmosis is remarkably distributed among (studied) domestic cats in Phayao, Thailand. Moreover, the virulent GRA6 allele was found to be circulated among domestic cats in this area. However, no significant correlation was observed between infection rates and different risk factors, which indicated that pet cats of any age, gender, or breed have similar risks of being infected with T. gondii. Our results further suggested that infective oocysts of T. gondii are widely distributed and that environmental contamination with these oocysts will introduce more risks of disease transmission to humans and other animals. Keywords: domestic cats, genotype, granule antigen 6, Toxoplasma gondii, toxoplasmosis.
Keywords: domestic cats, genotype, granule antigen 6, Toxoplasma gondii, toxoplasmosis.
How to cite this article: Suwancharoen C, Phuangsri C, Prakhammin K, and Japa O (2022) Molecular detection and dense granule antigen 6 genotyping of feline Toxoplasma gondii in Phayao, Thailand, Veterinary World, 15(9): 2309–2314.
Received: 03-06-2022 Accepted: 22-08-2022 Published online: 26-09-2022
Corresponding author: Ornampai Japa E-mail: ornampai.ja@up.ac.th
DOI: 10.14202/vetworld.2022.2309-2314
Copyright: Suwancharoen, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.