Vet World   Vol.15   September-2022  Article-26

Enhancement of curcumin level and hepatoprotective effect in rats through antioxidant activity following modification into nanosized particles

Background and Aim: Developing curcumin into nanosized particles is one of the approaches to overcome the limited use of curcumin. This study aimed to prepare curcumin into nanosized particles to increase the curcumin level in the rat's liver and hepatoprotective effect in rats.

Materials and Methods: Curcumin into nanosized particles formulated using ionic gelation method. Rats were divided into four groups (n = 6): Normal, negative, curcumin, and curcumin modified into nanosized particles were treated with 100 mg/ kg body weight orally for 14 days. Hepatic curcumin level was investigated using liquid chromatography with tandem mass spectrometry, antioxidant activity by malondialdehyde (MDA), and hepatoprotective effect by aspartate transaminase (AST), alanine transaminase (ALT), and histopathology.

Results: The curcumin level in the rat's liver in the curcumin group was 12.19 ng/mL, and that in those receiving modified into nanosized curcumin was 209.36 ng/mL. The MDA levels in the normal, negative, curcumin, and curcumin modified into nanosized particles groups were 1.88, 4.87, 3.38, and 1.04 nmol/L, respectively. The AST levels in these groups were 57.12, 130.00, 102.13, and 74.28 IU/L, and the ALT levels were 21.63, 61.97, 39.38, and 28.55 IU/L. The liver histopathology scoring showed that curcumin in nanosized particles was better than curcumin in degeneration of fat, lymphocyte infiltration, and necrosis.

Conclusion: There was a 17 times increase in curcumin level in the liver of rats treated with curcumin modified into nanosized particles. Curcumin modified into nanosized particles showed more significant improvement as antioxidant and hepatoprotector than curcumin. Keywords: antioxidant, curcumin modified into nanosized particles, hepatoprotective.

Keywords: antioxidant, curcumin modified into nanosized particles, hepatoprotective.

How to cite this article: Sandhiutami NMD, Dewi RS, Khairani S, and Putri RNA (2022) Enhancement of curcumin level and hepatoprotective effect in rats via antioxidant activity following modification into nanosized particles, Veterinary World, 15(9): 2323–2332.

Received: 04-05-2022  Accepted: 22-08-2022     Published online: 29-09-2022

Corresponding author: Ni Made Dwi Sandhiutami   E-mail: dwisandhiutami@univpancasila.ac.id

DOI: 10.14202/vetworld.2022.2323-2332

Copyright: Sandhiutami NMD, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.