Antimicrobial resistance and molecular characterization of Escherichia coli isolated from bovine mastitis samples in Nghe An province, Vietnam

Vet World Vol.16 April-2023 Article-11

Research Article

Veterinary World, 16(4): 743-751

https://doi.org/10.14202/vetworld.2023.743-751

Tran Trung My^1,2, Le Van Thien^2,3, Vu Duy Manh⁴, Bui Thi Phuong My¹, Dang Thi Mai Lan¹, Dang Xuan Binh¹, and Vu Minh Duc^5,6

1. Department of Animal Science and Veterinary Medicine, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen University, Quyet Thang, Thai Nguyen City, Vietnam.
2. Department of Quality Assurance, TH Dairy Institute, Nghia Son, Nghia Dan, Nghe An, Vietnam.
3. Department of Veterinary Medicine, The Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Vietnam.
4. TH Milk Food Joint Stock Company, Nghia Son, Nghia Dan, Nghe An, Vietnam.
5. Department of Agro-forestry Technology, College of Economics and Technology, Thai Nguyen University, Thinh Dan, Thai Nguyen City, Vietnam.
6. Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Background and Aim: Vietnam’s dairy sector is in its early phase of large-scale farming development. Therefore, mastitis in cows is always a concern to farm owners. This study aimed to determine the antimicrobial susceptibility, resistance, and virulence-related genes of Escherichia coli isolated from bovine mastitis in Nghe An province of Vietnam.

Materials and Methods: Fifty E. coli strains were isolated from the clinical cases and subjected to this study. All isolates were tested for antimicrobial susceptibility by the disk-diffusion method, as described by the Clinical and Laboratory Standards Institute. Antimicrobial and virulence genes were confirmed by polymerase chain reaction with specific primers.

Results: All isolates were resistant to lincomycin and sulfamethoxazole and sensitive to gentamicin, while other antimicrobials showed resistance from 2% to 90%. Multidrug resistance was confirmed in 46% of isolates, and none of them were identified as extended-spectrum beta-lactamase producers. From fifty strains tested for antimicrobial and virulence genes, six isolates harbored tetA, 6 tetB, 13 sul1, 15 sul2, 2 Intimin (eae), 1 iutA, and 3 stx2.

Conclusion: Antimicrobial and multidrug resistances are the main virulence factors of E. coli isolated from bovine mastitis in Vietnam. The virulence genes encoding adhesion, siderophore, Shiga-toxin-producing, and antimicrobials resistant were first reported in Vietnam with low prevalence and contributed to the pathogenesis. Keywords: antimicrobial resistance, bovine mastitis, Escherichia coli, virulence genes.

Keywords: antimicrobial resistance, bovine mastitis, Escherichia coli, virulence genes.

How to cite this article: My TT, Thien LV, Manh VD, My BTP, Lan DTM, Binh DX, and Duc VM (2023) Antimicrobial resistance and molecular characterization of

Received: 13-01-2023 Accepted: 06-03-2023 Published online: 13-04-2023

Corresponding author: E-mail: dangxuanbinh@tuaf.edu.vn

DOI: 10.14202/vetworld.2023.743-751

Copyright: My, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.