Open Access
Research (Published online: 13-04-2023)
11. Antimicrobial resistance and molecular characterization of Escherichia coli isolated from bovine mastitis samples in Nghe An province, Vietnam
Tran Trung My, Le Van Thien, Vu Duy Manh, Bui Thi Phuong My, Dang Thi Mai Lan, Dang Xuan Binh, and Vu Minh Duc
Veterinary World, 16(4): 743-751

Tran Trung My: Department of Animal Science and Veterinary Medicine, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen University, Quyet Thang, Thai Nguyen City, Vietnam; Department of Quality Assurance, TH Dairy Institute, Nghia Son, Nghia Dan, Nghe An, Vietnam.
Le Van Thien: Department of Quality Assurance, TH Dairy Institute, Nghia Son, Nghia Dan, Nghe An, Vietnam; Department of Veterinary Medicine, The Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Vietnam.
Vu Duy Manh: TH Milk Food Joint Stock Company, Nghia Son, Nghia Dan, Nghe An, Vietnam.
Bui Thi Phuong My: Department of Animal Science and Veterinary Medicine, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen University, Quyet Thang, Thai Nguyen City, Vietnam.
Dang Thi Mai Lan: Department of Animal Science and Veterinary Medicine, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen University, Quyet Thang, Thai Nguyen City, Vietnam.
Dang Xuan Binh: Department of Animal Science and Veterinary Medicine, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen University, Quyet Thang, Thai Nguyen City, Vietnam.
Vu Minh Duc: Department of Agro-forestry Technology, College of Economics and Technology, Thai Nguyen University, Thinh Dan, Thai Nguyen City, Vietnam; Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

doi: 10.14202/vetworld.2023.743-751

Article history: Received: 13-01-2023, Accepted: 06-03-2023, Published online: 13-04-2023

Corresponding authors: Dang Xuan Binh

E-mail: dangxuanbinh@tuaf.edu.vn

Citation: My TT, Thien LV, Manh VD, My BTP, Lan DTM, Binh DX, and Duc VM (2023) Antimicrobial resistance and molecular characterization of Escherichia coli isolated from bovine mastitis samples in Nghe An province, Vietnam, Veterinary World, 16(4): 743-751.
Abstract

Background and Aim: Vietnam’s dairy sector is in its early phase of large-scale farming development. Therefore, mastitis in cows is always a concern to farm owners. This study aimed to determine the antimicrobial susceptibility, resistance, and virulence-related genes of Escherichia coli isolated from bovine mastitis in Nghe An province of Vietnam.

Materials and Methods: Fifty E. coli strains were isolated from the clinical cases and subjected to this study. All isolates were tested for antimicrobial susceptibility by the disk-diffusion method, as described by the Clinical and Laboratory Standards Institute. Antimicrobial and virulence genes were confirmed by polymerase chain reaction with specific primers.

Results: All isolates were resistant to lincomycin and sulfamethoxazole and sensitive to gentamicin, while other antimicrobials showed resistance from 2% to 90%. Multidrug resistance was confirmed in 46% of isolates, and none of them were identified as extended-spectrum beta-lactamase producers. From fifty strains tested for antimicrobial and virulence genes, six isolates harbored tetA, 6 tetB, 13 sul1, 15 sul2, 2 Intimin (eae), 1 iutA, and 3 stx2.

Conclusion: Antimicrobial and multidrug resistances are the main virulence factors of E. coli isolated from bovine mastitis in Vietnam. The virulence genes encoding adhesion, siderophore, Shiga-toxin-producing, and antimicrobials resistant were first reported in Vietnam with low prevalence and contributed to the pathogenesis.

Keywords: antimicrobial resistance, bovine mastitis, Escherichia coli, virulence genes.