Vet World Vol.16 April-2023 Article-16
Research Article
Veterinary World, 16(4): 786-791
https://doi.org/10.14202/vetworld.2023.786-791
The first study on antimicrobial resistance of Staphylococcus aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, East Java, Indonesia
2. Department of Veterinary Science, Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia.
3. Department of Veterinary Science, Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia.
4. Department of Veterinary Science, Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia.
5. Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia.
Background and Aim: Raw goat milk is a highly nutritious dairy product and a suitable medium for the growth of antimicrobial-resistant Staphylococcus aureus, the leading cause of subclinical mastitis. This study aimed to investigate the resistance status of S. aureus which isolated from goat milk associated with subclinical mastitis cases in Siliragung Subdistrict, Banyuwangi District, East Java, Indonesia.
Materials and Methods: The S. aureus isolates were recovered from 258 raw goat milk samples from seven different dairy goat farms. Preliminary screening of subclinical mastitis using the California mastitis test, then samples with score +3 and +4 were taken for further isolation and identification, followed by a biochemical test to determine the S. aureus. Moreover, the bacteria susceptibility test against several antimicrobials was done using the disk diffusion method.
Results: Based on our findings, a total of 66 (25.58%) raw goat milk samples were tested positive for S. aureus, of which 36.36% were identified as multidrug-resistant. Moreover, S. aureus were also identified as resistant to penicillin (81.82%), ampicillin (65.15%), erythromycin (50.52%), and gentamicin (36.09%).
Conclusion: The prevalence of S. aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, Banyuwangi District, Indonesia, was recorded at 25.58%. Moreover, 36.36% of S. aureus isolates were categorized as resistant to three or more classes of antibiotics. The biosafety and biosecurity procedures during the milking process should be strengthened in dairy goat farms to prevent the transmission of antimicrobial resistance among animals, humans, and environments. Keywords: antimicrobial resistance, food security, infectious disease, public health, tropical disease.
Keywords: antimicrobial resistance, food security, infectious disease, public health, tropical disease.
How to cite this article: Praja RN, Yudhana A, Saputro AL, and Hamonangan JM (2023) The first study on antimicrobial resistance of
Received: 21-12-2022 Accepted: 08-03-2023 Published online: 18-04-2023
Corresponding author: E-mail: ratihnovitapraja@fkh.unair.ac.id
DOI: 10.14202/vetworld.2023.786-791
Copyright: Praja, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.