Characterization of polymorphisms in the follicle-stimulating hormone receptor and insulin-like growth factor-1 genes and their association with fertility traits in Jawa-Brebes cows

Vet World Vol.16 April-2023 Article-7

Research Article

Veterinary World, 16(4): 711-716

https://doi.org/10.14202/vetworld.2023.711-716

Slamet Hartanto^1,2, Agung Budiyanto¹, Rini Widayanti³, Erif Maha Nugraha Setyawan¹, and Imawan Daru Prasetya⁴

1. Department of Reproduction, Obstetrics, and Gynecology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
2. National Research and Innovation Agency (BRIN), Jakarta, Indonesia.
3. Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
4. Directorate General of Livestock and Animal Health, Ministry of Agriculture, Jakarta, Indonesia.

Background and Aim: The availability of fertility markers is crucial for maintaining, protecting, and improving the genetics of Jawa-Brebes (Jabres) cows. Follicle-stimulating hormone receptor (FSHR) and insulin-like growth factor-1 (IGF-1) play critical roles in female reproductive physiology. The single-nucleotide polymorphisms (SNPs) FSHR G-278A> and IGF-1 C-512T correlate with cows' fertility traits. This study aimed to identify these SNPs and their potential associations with fertility parameters in Jabres cows.

Materials and Methods: Samples were collected from 45 heads of multiparous Jabres cows aged 3–10 years with body condition scores of 2.5–5.0 on a 5-point scale in Brebes Regency, Java, Indonesia. These cows were assigned to fertile (n = 16) and infertile groups (n = 29). Polymerase chain reaction (PCR) was carried out for DNA amplification of FSHR G-278A and IGF-1 C-512T fragments. Restriction fragment length polymorphism-PCR with the restriction enzymes FaqI for the product of FSHR G-278A and SnaBI for the product of IGF-1 C-512T was used to identify SNPs.

Results: The FaqI enzyme cut the 211 bp DNA fragment of FSHR G-278A in all samples into two bands of 128 bp and 83 bp (GG genotype). Meanwhile, the genotyping of amplicon products of IGF-1 C-512T generated a single 249 bp fragment (CC genotype) in both groups.

Conclusion: The results showed that the FSHR G-278A/FaqI and IGF-1 C-512T/SnaBI loci were monomorphic in Jabres cows. Thus, neither FSHR G-278A/FaqI nor IGF-1 C-512T/SnaBI is a possible genetic marker for fertility in Jabres cows. Keywords: fecundity, genetic marker, Indonesian cow, restriction fragment length polymorphism-polymerase chain reaction.

Keywords: fecundity, genetic marker, Indonesian cow, restriction fragment length polymorphism-polymerase chain reaction.

How to cite this article: Hartanto S, Budiyanto A, Widayanti R, Setyawan EMN, and Prasetya ID (2023) Characterization of polymorphisms in the follicle-stimulating hormone receptor and insulin-like growth factor-1 genes and their association with fertility traits in Jawa-Brebes cows, Veterinary World, 16(4): 711-716.

Received: 28-11-2022 Accepted: 27-02-2023 Published online: 09-04-2023

Corresponding author: E-mail: hartanto.aisya@gmail.com and budiyanto@ugm.ac.id

DOI: 10.14202/vetworld.2023.711-716

Copyright: Hartanto, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.