Vet World   Vol.16   August-2023  Article-23

Screening and quantification of antibiotic residues in poultry products and feed in selected areas of Bangladesh

Background and Aim: Antibiotic residues in livestock farming have been identified as a potential cause of antimicrobial resistance in humans and animals. This study aimed to determine whether antibiotic residues were present in the chicken meat, eggs, feces, and feed collected from all four districts in the Mymensingh division of Bangladesh.

Materials and Methods: To detect antibiotic residues in the collected samples, qualitative thin-layer chromatography (TLC) and quantitative high-performance liquid chromatography (HPLC) were used. A total of 230 samples were analyzed for commonly used 11 antibiotics residue. Out of these, 40 meats and 40 feces samples were collected from broilers and layers, 30 egg samples from duck and layer, and 120 feed samples from both broilers and layers from the study area. Thin-layer chromatography was used for screening the presence of antibiotic residues; TLC-positive samples were then subjected to further HPLC analysis to determine the residue concentrations.

Results: Thin-layer chromatography analysis revealed that 23.5% of the tested samples contained residues from six different antibiotic classes (tetracyclines, quinolones, beta-lactams, sulfonamides, aminoglycosides, and macrolides). Thin-layer chromatography analysis showed that 35% and 25% of the meat samples were positive for residues from the broiler and layer, respectively. About 15% and 30% of layer and duck egg samples had positive residues, respectively. Out of 120 feed samples analyzed, about 15.8% had various antibiotic residues. In addition, feces samples from broilers and layers had 50% and 35% antibiotic residues, respectively. A total of 2.5% meat and 3.3% egg samples had antibiotic residues above the maximum residue limit (MRL). Based on the findings of this study, the highest percentage of oxytetracycline, followed by doxycycline and ciprofloxacin, were detected in feed samples, and oxytetracycline was detected in meat and egg samples.

Conclusion: This study clearly showed the misuse of antibiotics in the poultry sector in Bangladesh. Although antibiotic residues below the MRL level are suitable for human consumption, they may result in antimicrobial drug resistance to pathogens. Keywords: antibiotic residues, broiler, high-performance liquid chromatography, layer, maximum residue limit, thin-layer chromatography.

Keywords: antibiotic residues, broiler, high-performance liquid chromatography, layer, maximum residue limit, thin-layer chromatography.

How to cite this article: Sani AA, Rafiq K, Hossain MT, Akter F, Haque A, Hasan MI, Sachi S, Mustari A, Islam MZ, and Alam MM (2023) Screening and quantification of antibiotic residues in poultry products and feed in selected areas of Bangladesh, Veterinary World, 16(8): 1747-1754.

Received: 16-04-2023  Accepted: 04-08-2023     Published online: 28-08-2023

Corresponding author: Kazi Rafiq   E-mail: kazirafiq@bau.edu.bd

DOI: 10.14202/vetworld.2023.1747-1754

Copyright: Sani, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.