Effects of Solanum tuberosum L. ointment on second-degree burns in mice

Research (Published online: 16-12-2023)

7. Effects of Solanum tuberosum L. ointment on second-degree burns in mice

Carmen R. Silva-Correa, Galy P. Rosas-Cruz, Abhel A. Calderón-Peña, Víctor E. Villarreal-La Torre, Cinthya L. Aspajo-Villalaz, Julio A. Castañeda-Carranza, Deivy Y. Dionicio-Rosado, Ricardo M. Gómez-Arce, Cristhian N. Rodríguez-Silva, Jorge Del Rosario-Chávarri, and José L. Cruzado-Razco

Veterinary World, 16(12): 2440-2445

Carmen R. Silva-Correa: Department of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, Peru.

Galy P. Rosas-Cruz: Department of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, Peru.

Abhel A. Calderón-Peña: Department of Biological Chemistry and Animal Physiology, Faculty of Biological Sciences, National University of Trujillo, Peru.

Víctor E. Villarreal-La Torre: Department of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, Peru.

Cinthya L. Aspajo-Villalaz: Department of Biological Chemistry and Animal Physiology, Faculty of Biological Sciences, National University of Trujillo, Peru.

Julio A. Castañeda-Carranza: Professional Department of Statistics, Faculty of Physical Sciences and Mathematics, National University of Trujillo, Peru.

Deivy Y. Dionicio-Rosado: Professional Department of Statistics, Faculty of Physical Sciences and Mathematics, National University of Trujillo, Peru.

Ricardo M. Gómez-Arce: Professional Department of Statistics, Faculty of Physical Sciences and Mathematics, National University of Trujillo, Peru.

Cristhian N. Rodríguez-Silva: Department of Biological Chemistry and Animal Physiology, Faculty of Biological Sciences, National University of Trujillo, Peru.

Jorge Del Rosario-Chávarri: Department of Biological Chemistry and Animal Physiology, Faculty of Biological Sciences, National University of Trujillo, Peru.

José L. Cruzado-Razco: Department of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, Peru.

Background and Aim: Potato (Solanum tuberosum L.) is mainly characterized by its antioxidant and healing properties. Therefore, this study aimed to evaluate the effects of an ointment based on S. tuberosum L. “papa tumbay” on burns induced in Balb/c mice (Mus musculus).

Materials and Methods: The experimental animals were divided into four groups (n = 5/group) 48 h before second-degree burns were inducted. After epilating the loin areas of the mice and anesthetizing them with ketamine/xylazine (80 mg/kg/10 mg/kg) through intraperitoneal (i.p.) route, a round metal rod (0.7 cm in diameter) was placed on the depilated skin at a temperature of 100°C for 5 s. Group I was not given any treatment, Group II was treated with silver sulfadiazine (1%), and the other two groups (III and IV) were treated with the ointment formulated based on S. tuberosum L. “papa tumbay” at 1% and 2%, respectively. After performing the treatment for 21 days, the mice were euthanized using i.p. sodium pentobarbital (185 mg/kg) to obtain skin samples. The samples were preserved in 10% neutral-buffered formalin and subjected to histopathological analysis.

Results: We found statistically significant differences in the histopathological sections between the groups (p < 0.05). The abundant collagen and fibroblasts observed in the direction of the dermis in Groups III and IV indicate that the phytoconstituents present in the potato might promote the healing of the second-degree burns until day 21 of treatment.

Conclusion: Our findings showed that the ointments based on the ethanolic extracts of S. tuberosum L. “papa tumbay,” especially the 2% ointment, might accelerate the healing of second-degree burns induced in Balb/c mice.

Keywords: burn healing, herbal medicine, histopathology, ointment, potato, skin, Solanum tuberosum L.