Open Access
Research (Published online: 31-01-2023)
29. Methicillin-resistant Staphylococcus aureus isolates derived from humans and animals in Yogyakarta, Indonesia
Mulya Fitranda, Siti Isrina Oktavia Salasia, Osman Sianipar, Dion Adiriesta Dewananda, Adika Zhulhi Arjana, Fatkhanuddin Aziz, Madarina Wasissa, Fajar Budi Lestari, and Christin Marganingsih Santosa
Veterinary World, 16(1): 239-245

Mulya Fitranda: Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Siti Isrina Oktavia Salasia: Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Osman Sianipar: Department of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Dion Adiriesta Dewananda: Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Adika Zhulhi Arjana: Department of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Fatkhanuddin Aziz: Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Madarina Wasissa: Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Fajar Budi Lestari: Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Christin Marganingsih Santosa: Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

doi: www.doi.org/10.14202/vetworld.2023.239-245

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Article history: Received: 07-09-2022, Accepted: 19-12-2022, Published online: 31-01-2023

Corresponding author: Siti Isrina Oktavia Salasia

E-mail: isrinasalasia@ugm.ac.id

Citation: Fitranda M, Salasia SIO, Sianipar O, Dewananda DA, Arjana AZ, Aziz F, Wasissa M, Lestari FB, and Santosa CM (2023) Methicillin-resistant Staphylococcus aureus isolates derived from humans and animals in Yogyakarta, Indonesia, Veterinary World, 16(1): 239–245.
Abstract

Background and Aim: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain in veterinary and human medicine is a growing global problem. This study aimed to evaluate MRSA isolates of human and animal origin against various antibiotics in Yogyakarta, Indonesia.

Materials and Methods: The susceptibility test was carried out by the disk diffusion method using Mueller-Hinton agar against nine antibiotic disks. Methicillin-resistant S. aureus strains were genetically confirmed through mecA gene detection encoding for methicillin resistance by polymerase chain reaction.

Results: All 240 S. aureus strains isolated from animals and humans were resistant to penicillin G (P) (100% and 99%, respectively), followed by ampicillin (AMP), amoxicillin (AML), oxacillin (OX), erythromycin (E), clindamycin (DA), tetracycline (TE), gentamicin (GEN), and ciprofloxacin (CIP). Eighty-three MRSA strains were resistant to OX (100%), P (100%), AMP (99.27%), AML (95.52%), E (87.77%), TE (71.33%), DA (63.24%), GEN (38.81%), and CIP (26.87%).

Conclusion: The antimicrobial resistance pattern of S. aureus human isolates was similar to their animal counterpart, with 77.20% of MRSA strains classified as multidrug-resistant (MDR) bacteria. These findings indicate an increase in MDR S. aureus strains of animal origin in Yogyakarta, thus raising public health concerns about MRSA zoonotic spread.

Keywords: antimicrobial resistance, methicillin-resistant Staphylococcus aureus, multi-drug resistance, Staphylococcus aureus.