Vet World   Vol.16   January-2023  Article-29

Research Article

Veterinary World, 16(1): 239-245

https://doi.org/10.14202/vetworld.2023.239-245

Methicillin-resistant Staphylococcus aureus isolates derived from humans and animals in Yogyakarta, Indonesia

Mulya Fitranda1, Siti Isrina Oktavia Salasia1, Osman Sianipar2, Dion Adiriesta Dewananda1, Adika Zhulhi Arjana2, Fatkhanuddin Aziz3, Madarina Wasissa1, Fajar Budi Lestari3, and Christin Marganingsih Santosa1
1. Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
2. Department of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3. Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Background and Aim: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain in veterinary and human medicine is a growing global problem. This study aimed to evaluate MRSA isolates of human and animal origin against various antibiotics in Yogyakarta, Indonesia.

Materials and Methods: The susceptibility test was carried out by the disk diffusion method using Mueller-Hinton agar against nine antibiotic disks. Methicillin-resistant S. aureus strains were genetically confirmed through mecA gene detection encoding for methicillin resistance by polymerase chain reaction.

Results: All 240 S. aureus strains isolated from animals and humans were resistant to penicillin G (P) (100% and 99%, respectively), followed by ampicillin (AMP), amoxicillin (AML), oxacillin (OX), erythromycin (E), clindamycin (DA), tetracycline (TE), gentamicin (GEN), and ciprofloxacin (CIP). Eighty-three MRSA strains were resistant to OX (100%), P (100%), AMP (99.27%), AML (95.52%), E (87.77%), TE (71.33%), DA (63.24%), GEN (38.81%), and CIP (26.87%).

Conclusion: The antimicrobial resistance pattern of S. aureus human isolates was similar to their animal counterpart, with 77.20% of MRSA strains classified as multidrug-resistant (MDR) bacteria. These findings indicate an increase in MDR S. aureus strains of animal origin in Yogyakarta, thus raising public health concerns about MRSA zoonotic spread. Keywords: antimicrobial resistance, methicillin-resistant Staphylococcus aureus, multi-drug resistance, Staphylococcus aureus.

Keywords: antimicrobial resistance, methicillin-resistant Staphylococcus aureus, multi-drug resistance, Staphylococcus aureus.

How to cite this article: Fitranda M, Salasia SIO, Sianipar O, Dewananda DA, Arjana AZ, Aziz F, Wasissa M, Lestari FB, and Santosa CM (2023) Methicillin-resistant Staphylococcus aureus isolates derived from humans and animals in Yogyakarta, Indonesia, Veterinary World, 16(1): 239–245.

Received: 07-09-2022  Accepted: 19-12-2022     Published online: 31-01-2023

Corresponding author: Siti Isrina Oktavia Salasia   E-mail: isrinasalasia@ugm.ac.id

DOI: 10.14202/vetworld.2023.239-245

Copyright: Fitranda, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.