The potential of lactic acid bacteria isolated from ikan budu (fermented fish) to inhibit the growth of pathogenic fungi and detoxify aflatoxin B1

Research (Published online: 04-07-2023)

1. The potential of lactic acid bacteria isolated from ikan budu (fermented fish) to inhibit the growth of pathogenic fungi and detoxify aflatoxin B1

Yetti Marlida, Nurmiati Nurmiati, Husmaini Husmaini, Nurul Huda, Lili Anggraini, and Laily Rinda Ardani

Veterinary World, 16(7): 1373-1379

Yetti Marlida: Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.

Nurmiati Nurmiati: Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang City, West Sumatra 25175, Indonesia.

Husmaini Husmaini: Department of Animal Production, Faculty of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.

Nurul Huda: Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, 90509, Sandakan, Sabah, Malaysia.

Lili Anggraini: Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.

Laily Rinda Ardani: Graduate Program of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.

doi: 10.14202/vetworld.2023.1373-1379

Article history: Received: 04-03-2023, Accepted: 23-05-2023, Published online: 04-07-2023

Corresponding author: Yetti Marlida

E-mail: yettimarlida@ansci.unand.ac.id

Citation: Marlida Y, Nurmiati N, Husmaini H, Huda N, Anggraini L, and Ardani LR (2023) The potential of lactic acid bacteria isolated from ikan budu (fermented fish) to inhibit the growth of pathogenic fungi and detoxify aflatoxin B1, Veterinary World, 16(7): 1373-1379.

Abstract

Background and Aim: Market demand for safe feed and food supply and consumer preferences for safe and healthy products are increasing. Control measures to counter threats to the feed supply need to be implemented as early as possible to prevent economic losses. Mycotoxins produced by certain groups of fungi are a problem that can disrupt the feed supply or pose a threat to the health of animals and humans. Biological control to detoxify contaminated feed ingredients can be carried out on a large scale economically. For example, lactic acid bacteria (LAB) can act as biological agents for eliminating mycotoxins. This study aimed to clarify the value of screening LAB to inhibit Aspergillus flavus> growth and detoxify aflatoxin B1 (AFB1).

Materials and Methods: In this study, using a completely randomized design with three replications, five isolates of LAB (LA.1, LA.6, LA.8, LA.12, and LA.22) along with their supernatants were tested qualitatively and quantitatively for their ability to counter mycotoxins using A. flavus and corn kernels. The isolates with the best activity were identified by sequencing 16S rDNA.

Results: The results showed that the five LAB isolates can inhibit the growth of A. flavus and detoxify AFB1. Among these isolates, LA.12 showed the best performance, followed by LA.22, LA.8, LA.6, and then LA.1. The sequencing results confirmed that LA.12 was Lactobacillus harbinensis strain 487.

Conclusion: All of the isolates in this study have the potential as biological agents for detoxifying AFB1, with isolate LA.12 appearing to be the most promising biodetoxification agent for feed (AFB1 in corn) based on its ability to inhibit pathogenic fungi.

Keywords: biodetoxification, feedstuffs, lactic acid bacteria, pathogenic fungi.