Review (Published online: 05-06-2023)
6. Realistic aspects behind the application of the rat model of chemically-induced mammary cancer: Practical guidelines to obtain the best results
Jéssica Silva, Ana I. Faustino-Rocha, José Alberto Duarte, and Paula A. Oliveira
Veterinary World, 16(6): 1222-1230
Jéssica Silva: Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Vila Real, Portugal; Institute for Innovation, Capacity Building and Sustainability of Agri-food Production (Inov4Agro), Vila Real, Portugal.
Ana I. Faustino-Rocha: Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Vila Real, Portugal; Institute for Innovation, Capacity Building and Sustainability of Agri-food Production (Inov4Agro), Vila Real, Portugal; Department of Zootechnics, School of Sciences and Technology, University of Évora, Portugal; Comprehensive Health Research Center, University of Évora, Évora, Portugal.
José Alberto Duarte: Research Center for Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, Porto, Portugal; Toxicology Research Unit (TOXRUN), Advanced Polytechnic and University Cooperative (CESPU), Gandra, Portugal.
Paula A. Oliveira: Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Vila Real, Portugal; Institute for Innovation, Capacity Building and Sustainability of Agri-food Production (Inov4Agro), Vila Real, Portugal; Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal.
doi: 10.14202/vetworld.2023.1222-1230
Article history: Received: 22-02-2023, Accepted: 04-05-2023, Published online: 05-06-2023
Corresponding author: Paula A. Oliveira
E-mail: pamo@utad.pt
Citation: Silva J, Faustino-Rocha AI, Duarte JA, and Oliveira PA (2023) Realistic aspects behind the application of the rat model of chemically-induced mammary cancer: Practical guidelines to obtain the best results, Veterinary World, 16(6): 1222-1230.
Abstract
Cancer is one of the most important public health problems worldwide. Despite the great contribution of in-vitro studies for biomedical research, animals are essential to study diseases’ biopathology and diagnosis, and searching for new preventive and therapeutic strategies. Breast cancer is currently the most common cancer globally, accounting for 12.5% of all new annual cancer cases worldwide. Although the rat model of mammary cancer chemically-induced is widely used to study this disease, there is a lack of standardization in procedures for cancer induction, sample collection, and analysis. Therefore, it is important to provide a practical guide for researchers aiming to work with this model to make the analysis of results more uniform. Thus, in this review, we provide the researchers with a detailed step-by-step guide to implement a rat model of mammary cancer, based on our wide experience in this field, to obtain the best results, maximum throughput of each experiment, and easy comparison among researches.
Keywords: carcinogenesis, guide, modeling, rodents, tips.