Flow cytometric evaluation of CD4+ and CD8+ T-cell in IPB-D2 chickens with different Newcastle disease antibody titers level

Vet World Vol.16 May-2023 Article-31

Research Article

Veterinary World, 16(5): 1161-1164

https://doi.org/10.14202/vetworld.2023.1161-1164

Dwi Lestari¹, Sri Murtini², Niken Ulupi³, Asep Gunawan³, and Cece Sumantri³

1. Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia.
2. Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia.
3. Department of Animal Disease and Veterinary Public Health, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, Indonesia.

Background and Aim: IPB-D2 chickens are selected from IPB-D1 due to their disease-resistance characteristics. One-way to evaluate the strength of a chicken’s immune system is by examining the number of circulating T lymphocytes. This assessment can be conducted using a modern analytic method called flow cytometry which relies on monoclonal antibodies to detect the relative proportions of each cell and measure the quality and quantity of biological and physical features of cells, including specific membrane or intracellular glycoprotein markers. Therefore, this study aimed to evaluate the population of lymphocytes, cluster of differentiation (CD)4+ and CD8+ in IPB-D2 chickens.

Materials and Methods: Flow cytometry was used to evaluate the population of lymphocytes, CD4+, and CD8+ in IPB-D2 chickens. The data obtained in this study were analyzed by Minitab, and the mean values were compared using a t-test.

Results: The lymphocytes, CD4+, and CD8+ populations of IPB-D2 chicken with high Newcastle disease (ND) antibody titers were 65.04%, 10.53%, and 5.47%. Meanwhile, this breed, with low ND antibody titers had lymphocytes CD4+ and CD8+ population of 57.19%, 8.40%, and 4.11 %. The comparison of CD4+ and CD8+ populations in chickens with high and low ND antibody titers was 1.92 and 2.04, respectively.

Conclusion: IPB-D2 chickens with high ND antibody titers exhibited increased lymphocyte, CD4+, and CD8+ cell populations in comparison to those with low ND antibody titers. However, the high ND antibody titer group had a lower CD4+/CD8+ ratio. Keywords: cluster of differentiation 4+, cluster of differentiation 8+, flow cytometry, IPB-D2 chicken, lymphocytes.

Keywords: cluster of differentiation 4+, cluster of differentiation 8+, flow cytometry, IPB-D2 chicken, lymphocytes.

How to cite this article: Lestari D, Murtini S, Ulupi N, Gunawan A, and Sumantri C (2023) Flow cytometric evaluation of CD4+ and CD8+ T-cell in IPB-D2 chickens with different Newcastle disease antibody titers level, Veterinary World, 16(5): 1161-1164.

Received: 30-01-2023 Accepted: 02-05-2023 Published online: 30-05-2023

Corresponding author: E-mail: ceces@apps.ipb.ac.id

DOI: 10.14202/vetworld.2023.1161-1164

Copyright: Lestari, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.