Vet World Vol.16 November-2023 Article-24
Research Article
Veterinary World, 16(11): 2366-2373
https://doi.org/10.14202/vetworld.2023.2366-2373
Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin
2. Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
3. Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
4. Laboratory Animal Science Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
5. Kasetsart University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
6. Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
7. Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
8. Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Background and Aim: Asthma, a chronic disease affecting humans and animals, has recently become increasingly prevalent and steadily widespread. The alternative treatment of asthma using helminth infections or helminth-derived immunomodulatory molecules (IMs) has been evaluated and demonstrated significant amelioration of disease severity index in vitro and in vivo. Trichinella spiralis, a parasitic nematode and its IMs, elicits a potential to relieve asthma and other immune-related disorders. In this study, we investigated the immunomodulatory function of recombinant T. spiralis novel cystatin (rTsCstN) in ameliorating acute inflammatory asthma disorders in a murine model.
Materials and Methods: Female BALB/c mice were sensitized using intraperitoneal injection of ovalbumin (OVA)/alum and subsequently challenged with intranasal administration of OVA alone or OVA + rTsCstN for 3 consecutive days, producing OVA-induced allergic asthma models. To evaluate the therapeutic efficacy of rTsCstN, the inflammatory cells and cytokines in bronchoalveolar lavage fluid (BALF) and OVA-specific immunoglobulin E levels in serum were assessed. Histological alterations in the lung tissues were determined by hematoxylin and eosin (H&E) staining and eventually scored for the extent of inflammatory cell infiltration.
Results: The asthmatic mouse models challenged with OVA + rTsCstN demonstrated a significant reduction of eosinophils (p < 0.01), macrophages (p < 0.05), and cytokines tumor necrosis factor-α (p < 0.05) and interferon (IFN)-γ (p < 0.05) in BALF when compared with the mice challenged with OVA alone. However, the levels of interleukin (IL)-4 and IL-10 remained unchanged. Histological examination revealed that mice administered OVA + rTsCstN were less likely to have inflammatory cell infiltration in their perivascular and peribronchial lung tissues than those administered OVA alone.
Conclusion: Recombinant T. spiralis novel cystatin demonstrated immunomodulatory effects to reduce severe pathogenic alterations in asthma mouse models, encouraging a viable alternative treatment for asthma and other immunoregulatory disorders in humans and animals in the future. Keywords: asthma, immunomodulatory molecule, recombinant Trichinella spiralis novel cystatin, Trichinella spiralis.
Keywords: asthma, immunomodulatory molecule, recombinant Trichinella spiralis novel cystatin, Trichinella spiralis.
How to cite this article: Thammasonthijarern N, Boonnak K, Reamtong O, Krasae T, Thankansakul J, Phongphaew W, Ampawong S, and Adisakwattana P (2023) Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin, Veterinary World, 16(11): 2366-2373.
Received: 21-08-2023 Accepted: 23-10-2023 Published online: 27-11-2023
Corresponding author: Poom Adisakwattana E-mail: poom.adi@mahidol.edu
DOI: 10.14202/vetworld.2023.2366-2373
Copyright: Thammasonthijarern, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.