Open Access
Research (Published online: 21-09-2023)
18. Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia
M. Thoriq Ihza Farizqi, Mustofa Helmi Effendi, R. Tatang Santanu Adikara, Ira Sari Yudaniayanti, Giovanni Dwi Syahni Putra, Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Otto Sahat Martua Silaen, Safira Ramadhani, Saumi Kirey Millannia, Sergius Erikson Kaben, and Yusac Kristanto Khoda Waruwu
Veterinary World, 16(9): 1917-1925

M. Thoriq Ihza Farizqi: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Mustofa Helmi Effendi: Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
R. Tatang Santanu Adikara: Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Ira Sari Yudaniayanti: Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabay, 60115, East Java, Indonesia.
Giovanni Dwi Syahni Putra: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Aswin Rafif Khairullah: Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Shendy Canadya Kurniawan: Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research. Wageningen, 6708 PB, Netherlands.
Otto Sahat Martua Silaen: Department of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6 Senen, Jakarta, 10430, Indonesia.
Safira Ramadhani: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Saumi Kirey Millannia: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Sergius Erikson Kaben: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.
Yusac Kristanto Khoda Waruwu: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.

doi: 10.14202/vetworld.2023.1917-1925

Article history: Received: 22-06-2023, Accepted: 29-08-2023, Published online: 21-09-2023

Corresponding author: Mustofa Helmi Effendi

E-mail: mhelmieffendi@gmail.com

Citation: Farizqi MTI, Effendi MH, Adikara RTS, Yudaniayanti IS, Putra GDS, Khairullah AR, Kurniawan SC, Silaen OSM, Ramadhani S, Millannia SK, Kaben SE, and Waruwu YKK (2023) Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia, Veterinary World, 16(9): 1917-1925.
Abstract

Background and Aim: Escherichia coli causes a bacterial illness that frequently affects cats. Diseases caused by E. coli are treated using antibiotics. Because of their proximity to humans, cats possess an extremely high risk of contracting antibiotic resistance genes when their owners touch cat feces containing E. coli that harbor resistance genes. This study was conducted to identify multidrug-resistant E. coli and extended-spectrum β-lactamase (ESBL)-producing genes from cat rectal swabs collected at Surabaya City Veterinary Hospital to determine antibiotic sensitivity.

Materials and Methods: Samples of cat rectal swabs were cultured in Brilliant Green Bile Lactose Broth medium and then streaked on eosin methylene blue agar medium for bacterial isolation, whereas Gram-staining and IMViC tests were conducted to confirm the identification results. The Kirby–Bauer diffusion test was used to determine antibiotic sensitivity, and the double-disk synergy test was used to determine ESBL-producing bacteria. Molecular detection of the genes TEM and CTX-M was performed using a polymerase chain reaction.

Results: Based on morphological culture, Gram-staining, and biochemical testing, the results of sample inspection showed that of the 100 cat rectal swab samples isolated, 71 (71%) were positive for E. coli. Furthermore, 23 E. coli isolates (32.39%) demonstrated the highest resistance to ampicillin. Four isolates were confirmed to be multidurg-resistant and ESBL-producing strains. Molecular examination revealed that three E. coli isolates harbored TEM and CTX-M.

Conclusion: In conclusion, pet owners must be educated on the use of antibiotics to improve their knowledge about the risks of antibiotic resistance.

Keywords: cat, Escherichia coli, extended-spectrum β-lactamase, multidrug resistance, public health.