Vet World Vol.16 September-2023 Article-24
Research Article
Veterinary World, 16(9): 1974-1984
https://doi.org/10.14202/vetworld.2023.1974-1984
Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)
2. Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Senolowo, Yogyakarta 55281, Indonesia.
3. Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna, Caturtunggal, Yogyakarta 55281, Indonesia.
4. Laboratory of Aquatic Animal Health and Therapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
5. Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, Indonesia.
Background and Aim: The high diversity of Aeromonas spp. results in various pathogenicity levels. This group of bacteria causes a serious disease named motile Aeromonas septicemia (MAS) in catfish (Clarias spp.). This study aimed to characterize the species and virulence gene diversity of Aeromonas spp. isolated from diseased catfish.
Materials and Methods: Nine Aeromonas spp. were isolated from infected catfish cultivated in Java, Indonesia, and they were identified at the phenotypic and molecular levels (16S rDNA). The virulence genes assessed included aer/haem, alt, ast, flaA, lafA, and fstA.
Results: Phylogenetic analysis identified nine isolates of Aeromonas spp.: Aeromonas hydrophila (11.11%), Aeromonas caviae (11.11%), Aeromonas veronii bv. veronii (44.44%), and Aeromonas dhakensis (33.33%). Virulence genes, such as aer/haem, alt, ast, flaA, lafA, and fstA, were detected in all isolates at frequencies of approximately 100%, 66.67%, 88.89%, 100%, 55.56%, and 66.67%, respectively. This study is the first report on A. dhakensis recovered from an Indonesian catfish culture. Furthermore, our study revealed the presence of A. veronii bv veronii, a biovar that has not been reported before in Indonesia.
Conclusion: This finding confirms that MAS was caused by multiple species of Aeromonas, notably A. dhakensis and A. veronii bv veronii, within Indonesian fish culture. The presence of these Aeromonas species with multiple virulence genes poses a significant threat to the freshwater aquaculture industry. Keywords: 16S rDNA, aeromoniasis, motile Aeromonas septicemia, pathogenicity, phenotype, phylogenetic.
Keywords: 16S rDNA, aeromoniasis, motile Aeromonas septicemia, pathogenicity, phenotype, phylogenetic.
How to cite this article: Mulia DS, Pratiwi R, Asmara W, Azzam-Sayuti M, Yasin ISM, and Isnansetyo A (2023) Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.), Veterinary World, 16(9): 1974-1984.
Received: 11-05-2023 Accepted: 31-08-2023 Published online: 23-09-2023
Corresponding author: Alim Isnansetyo E-mail: isnansetyo@ugm.ac.id
DOI: 10.14202/vetworld.2023.1974-1984
Copyright: Mulia, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.