Molecular typing methods to characterize Brucella spp. from animals: A review

Vet World Vol.17 August-2024 Article - 14

Review Article

Veterinary World, 17(8): 1778-1788

https://doi.org/10.14202/vetworld.2024.1778-1788

Aida Daugaliyeva¹, Saule Daugaliyeva², Nazerke Kydyr¹, and Simone Peletto³

1. LLP “Kazakh Research Institute for Livestock and Fodder Production,” St. Zhandosova 51, Almaty 050035, Kazakhstan.
2. LLP “Scientific Production Center of Microbiology and Virology,” Bogenbay Batyr Str. 105, Almaty 050010, Kazakhstan.
3. Experimental Zooprofilactic Institute of Piedmont, Liguria and Aosta Valley, Via Bologna 148, 10154 Turin, Italy.

Brucellosis is an infectious disease of animals that can infect humans. The disease causes significant economic losses and threatens human health. A timely and accurate disease diagnosis plays a vital role in the identification of brucellosis. In addition to traditional diagnostic methods, molecular methods allow diagnosis and typing of the causative agent of brucellosis. This review will discuss various methods, such as Bruce-lаdder, Suiladder, high-resolution melt analysis, restriction fragment length polymorphism, multilocus sequence typing, multilocus variable-number tandem repeat analysis, and whole-genome sequencing single-nucleotide polymorphism, for the molecular typing of Brucella and discuss their advantages and disadvantages.

Keywords: Brucella, molecular typing, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, single-nucleotide polymorphisms, whole-genome sequencing.

How to cite this article: Daugaliyeva A, Daugaliyeva S, Kydyr N, and Peletto S (2024) Molecular typing methods to isolate Brucella spp. from animals: A review, Veterinary World, 17(8): 1778-1788.

Received: 2024-06-02 Accepted: 2024-07-18 Published online: 2024-08-13

Corresponding author: Saule Daugaliyeva E-mail: saule.daugalieva@mail.ru

DOI: 10.14202/vetworld.2024.1778-1788

Copyright: Daugaliyeva, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.