The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality

Vet World Vol.17 December-2024 Article - 25

Research Article

Veterinary World, 17(12): 2950-2956

https://doi.org/10.14202/vetworld.2024.2950-2956

Wilasinee Inyawilert^1,2, Yu-Jing Liao³, Oswald Nfor Ndi⁴, Koranit Pradithera¹, Atchawut Saengtun¹, Sureeporn Saengwong⁵, Payungsuk Intawicha⁵, Kunlayaphat Wuthijaree¹, Vorawatt Hanthongkul⁶, Kaikaew Kamdee⁶, Anurak Khieokhajonkhet^1,2, Chalothon Amporn⁷, Attapol Tiantong⁸, and Chompunut Lumsangkul⁹

1. Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.
2. The Center for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand.
3. Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture, Tainan, Taiwan.
4. Department of Public Health, Institute of Public Health, Chung Shan Medical University, Taichung, Taiwan.
5. Program of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand.
6. Phitsanulok Artificial Insemination and Biotechnology Research Center, Phitsanulok, Thailand.
7. Department of Veterinary Technology, Kalasin University, Kalasin, Thailand.
8. Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi IT Campus, Cha-Am, Phetchaburi, Thailand.
9. Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.

Background and Aim: The success of semen cryopreservation relies on several aspects, including breed, age, season, collection method, extender composition, cooling rate, equilibration period, freezing rate, and thawing rate. This study aimed to investigate the effects of cooling and equilibration duration, as well as the addition of antioxidants to the semen extender, on the cryopreservation of swamp buffalo semen.

Materials and Methods: Semen collected from swamp buffalo bulls was subjected to four different conditions: (T1) 2-h cooling and 2-h equilibration, (T2) 1.5-h cooling and 1.5-h equilibration, (T3) 1-h cooling and 1-h equilibration, and (T4) 0.5-h cooling and 0.5-h equilibration. Spermatozoa motility was evaluated using a computer-assisted semen analyzer. Moreover, this study also investigated the effect of antioxidant supplementation during cryopreservation using tris-citrate egg yolk extenders enriched with various antioxidants: Control (Con), 1 mM melatonin (ML), 0.5 mM gamma-oryzanol (GO), 10 μM canthaxanthin (CX), 1 mM melatonin + 0.5 mM gamma-oryzanol (ML + GO), and 1 mM melatonin + 10 μM canthaxanthin (ML + CX).

Results: Results showed that the (T1) 2-h cooling and 2-h equilibration and (T2) 1.5-h cooling and 1.5-h equilibration groups achieved higher progressive motility than the (T3) 1-h cooling and 1-h equilibration and (T4) 0.5-h cooling and 0.5-h equilibration groups. The ML-treated group exhibited superior progressive motility and total motility.

Conclusion: The optimal approach for cryopreserving swamp buffalo bull semen involves a 1.5-h cooling period followed by a 1.5-h equilibration period, with the incorporation of ML into the semen extender.

Keywords: antioxidant, cryopreservation, equilibration, semen, and swamp buffalo.

How to cite this article: Inyawilert W, Liao Y, Ndi ON, Pradithera K, Saengtun A, Saengwong S, Intawicha P, Wuthijaree K, Hanthongkul V, Kamdee K, Khieokhajonkhet A, Amporn C, Tiantong A, and Lumsangkul C (2024) The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality, Veterinary World, 17(12): 2950-2956.

Received: 2024-08-13 Accepted: 2024-11-26 Published online: 2024-12-26

Corresponding author: Wilasinee Inyawilert E-mail: wilasineei@nu.ac.th

DOI: 10.14202/vetworld.2024.2950-2956

Copyright: Inyawilert, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.