Vet World Vol.17 June-2024 Article - 23
Research Article
Veterinary World, 17(6): 1370-1384
https://doi.org/10.14202/vetworld.2024.1370-1384
Effects of house-cultivated edible bird’s nest on immunoglobulin and cytokine release in vitro
2. Centre for Biomedical and Nutrition Mel June Choong, Hemaniswarri Dewi Dewadas, Lay Cheng Lim, Sheela Devi Sukuru, Chee Hong Tan, Soon Keng Cheong, and Yang Mooi LimResearch, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Kampar, 31900, Perak, Malaysia.
3. Department of Business and Public Administration, Faculty of Business and Finance, Universiti Tunku Abdul Rahman, Jalan Universiti, Kampar, 31900, Perak, Malaysia.
4. Department of Life Sciences, School of Pharmacy, International Medical University, 57000 Kuala Lumpur, Malaysia.
5. Department of Nursing, M. Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, 43000 Kajang, Selangor, Malaysia.
6. Inbit Biotech Sdn. Bhd., No. 8, Lorong University B, Seksyen 16, 46350 Petaling Jaya, Selangor, Malaysia.
7. Department of Medicine, M. Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, 43000 Kajang, Selangor, Malaysia.
8. Department of Pre-clinical Sciences, M. Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, 43000 Kajang, Selangor, Malaysia.
Background and Aim: Edible bird’s nest (EBN) is known as the “Caviar of the East” because of its high nutritional and medicinal values. Nevertheless, its effect on human immunity is yet to be explored. This study examined the effects of EBN’s aqueous extract (EBNE) on human immunity through the modular immune in vitro construct (MIMIC) model consisting of peripheral tissue equivalent (PTE) and lymphoid tissue equivalent (LTE) modules.
Materials and Methods: One hundred twenty mL of full blood was obtained from four healthy human volunteers. The human immune system was simulated using an in vitro model, called MIMIC. Under EBNE treatment, monocyte transendothelial migration through reversed endothelial layers was observed. Using PTE and LTE modules, monocytes were differentiated into dendritic cells with lipopolysaccharide, then co-cultured with T- and B-cells for cytokine and immunoglobulin (Ig) production. The human cytokine array G2000 and quantitative human Ig isotyping array were used to identify the cytokine profile and Ig isotypes, respectively.
Results: IgE, IgA, and IgG3 levels were significantly raised by EBNE. These cytokines, including brain-derived neurotrophic factor, ciliary neurotrophic factor, glial cell line-derivative neurotrophic factor, insulin-like growth factor 1, and insulin-like growth factor binding protein 4, were generated.
Conclusion: For the first time, this work uses a MIMIC model to illustrate the impact of EBNE on human immune response. This new understanding of EBN’s immunoregulatory effect allows for further exploration of how EBN interacts with the human immune system.
Keywords: cytokines, edible bird’s nest, immunoglobulins, modular immune in vitro construct model, swiftlets.
How to cite this article: Choong MJ, Dewadas HD, Lim LC, Sukuru SD, Tan CH, Cheong SK, and Lim YM (2024) Effects of house-cultivated edible bird’s nest on immunoglobulin and cytokine release in vitro, Veterinary World, 17(6): 1370–1384.
Received: 2024-01-04 Accepted: 2024-05-14 Published online: 2024-06-28
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.1370-1384
Copyright: Choong, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
