Vet World Vol.17 June-2024 Article - 3
Research Article
Veterinary World, 17(6): 1202-1209
https://doi.org/10.14202/vetworld.2024.1202-1209
Occurrence of antimicrobial-resistant bovine mastitis bacteria in Sakon Nakhon, Thailand
2. Program in Animal Science, Faculty of Agricultural Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon 47000, Thailand.
3. Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
4. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Pathum Thani 12120, Thailand.
Background and Aim: Bovine mastitis is an inflammation of the mammary gland of dairy cattle that causes economic losses due to poor quantity and quality of milk. The extensive or incorrect use of antibiotics has increased in the veterinary field, leading to the emergence of antibiotic-resistant pathogens worldwide. This study aimed to investigate bovine mastitis bacterial pathogens in Sakon Nakhon, Thailand.
Materials and Methods: A total of 35 dairy farms were screened for clinical and subclinical mastitis using the California Mastitis Test and clinical examination. Polymerase chain reaction was used to characterize bacterial species-induced mastitis (380 isolates) in cattle and antimicrobial resistance genes, and disk diffusion and broth microdilution were used to characterize antimicrobial susceptibility.
Results: The prevalence of Staphylococcus epidermidis (38.10%; 32/84)-induced mastitis in cattle was considerably high, followed by Streptococcus agalactiae (33.33%), Streptococcus uberis (25%), Klebsiella pneumoniae (8.33%), and Staphylococcus aureus (4.76%). In this study, Staphylococcus spp. isolates demonstrated 100% susceptibility to cefoxitin, and no antibiotic-resistance genes were identified. Tetracycline (TET) and macrolide-resistant genes of Streptococcus spp. revealed that tetM was predominant in 55.63% (79/142), followed by tetS + erm(B) (16.90%). Antibiotic susceptibility tests revealed the following resistance profiles to bacterial species: TET (85.92%), clindamycin (29.58%), erythromycin (15.49%), levofloxacin (14.08%), and penicillin (0%). Gram-negative bacterial isolates (K. pneumoniae [8.33%], Klebsiella variicola [2.38%], Klebsiella quasipneumoniae [1.19%], and Escherichia coli [1.19%]) were recovered and still susceptible to meropenem (100%), ceftazidime (97.06%), ceftriaxone (79.41%), and ciprofloxacin (79.41%).
Conclusion: This result suggested that mastitis pathogens in this area were susceptible to most antimicrobials, with the exception of streptococci against TET. In this study, limited data were available including one from small-holder dairy farms and study only dairy farms in Sakon Nakhon, Thailand. So, more farms should be included in the future studies.
Keywords: antimicrobial resistance, bovine mastitis, genotype, phenotype, Thailand.
How to cite this article: Camsing A, Phetburom N, Chopjitt P, Pumhirunroj B, Patikae P, Watwiengkam N, Yongkiettrakul S, Kerdsin A, and Boueroy P (2024) Occurrence of antimicrobial-resistant bovine mastitis bacteria in Sakon Nakhon, Thailand, Veterinary World, 17(6): 1202-1209.
Received: 2024-02-16 Accepted: 2024-05-08 Published online: 2024-06-02
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.1202-1209
Copyright: Camsing, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
