Therapeutic efficacy of Centratherum anthelminticum in subclinical mastitis: A biochemical and hematological assessment in lactating cattle

Vet World Vol.18 June-2025 Article - 31

Research Article

Veterinary World, 18(6): 1741-1747

https://doi.org/10.14202/vetworld.2025.1741-1747

Muhammad Adil¹

, Farrah Deeba¹

, Muhammad Tariq²

, Muhammad Usman³

, Saba Saeed⁴

, Eliana Ibáñez-Arancibia^5,6,7

, Patricio De los Ríos-Escalante^7,8

, and Muhammad Safdar⁹

1. Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.

2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.

3. Department of Basic Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-Campus Narowal, Pakistan.

4. Department of Zoology, The Government Sadiq College Women University, 63100, Bahawalpur, Punjab, Pakistan.

5. PhD Program in Sciences Mentioning Applied Molecular and Cell Biology, La Frontera University, Temuco, Chile.

6. Laboratory of Engineering, Biotechnology and Applied Biochemistry, Department of Chemical Engineering, Faculty of Engineering and Science, La Frontera University, Temuco, Chile.

7. Department of Biological and Chemical Sciences, Faculty of Natural Resources, Catholic University of Temuco, Temuco, Chile.

8. Nucleus of Environmental Sciences, Faculty of Natural Resources, Catholic University of Temuco, Temuco, Chile.

9. Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, 63100, Bahawalpur, Pakistan.

Background and Aim: Subclinical mastitis (SCM) in dairy cattle significantly compromises milk quality, animal health, and farm profitability, often remaining undetected due to the absence of clinical signs. The increasing antimicrobial resistance associated with conventional treatments highlights the need for effective alternatives. This study aimed to evaluate the therapeutic potential of Centratherum anthelminticum (CA), alone and in combination with tylosin, in managing SCM in lactating cows.

Materials and Methods: Fifteen California mastitis test-positive cows were randomly divided into three groups (n = 5/group). Group A received tylosin (18 mg/kg intramuscular), Group B received CA (120 g orally), and Group C received both treatments. Milk samples were analyzed pre- and post-treatment for somatic cell count (SCC), pH, electrical conductivity, fat, protein, lactose, and solids-not-fat (SNF) content. Hematological parameters, including red blood cell (RBC), white blood cell, hemoglobin, packed cell volume (PCV), and lymphocyte percentages, were evaluated alongside oxidative stress markers – total antioxidant capacity (TAC) and total oxidative stress (TOS).

Results: Significant post-treatment reductions in SCC, pH, and conductivity were observed in all groups. Group C exhibited the most pronounced improvements in lactose, fat, and SNF, with no change in protein. Group B demonstrated the highest TAC increase and TOS reduction, affirming CA’s antioxidative potential. Hematological evaluations revealed systemic improvements post-treatment, particularly in RBC and PCV levels. Group B also showed increased lymphocyte counts, further indicating immunomodulatory effects.

Conclusion: CA exhibits considerable therapeutic potential in managing SCM, especially when combined with tylosin. Its antioxidative and immunomodulatory effects may enhance udder health and milk quality while reducing reliance on antibiotics. Future large-scale studies are warranted to confirm these findings and explore CA’s integration into sustainable mastitis management strategies.

Keywords: antioxidant capacity, Centratherum anthelminticum, dairy cattle, milk quality, oxidative stress, somatic cell count, subclinical mastitis, tylosin.

How to cite this article: Adil M, Deeba F, Tariq M, Usman M, Saeed S, Ibáñez-Arancibia E, Ríos-Escalante PD, and Safdar M (2025) Therapeutic efficacy of Centratherum anthelminticum in subclinical mastitis: A biochemical and hematological assessment in lactating cattle, Veterinary World, 18(6): 1741–1747.

Received: 02-12-2024 Accepted: 26-05-2025 Published online: 26-06-2025

Corresponding author: Farrah Deeba and Muhammad Tariq E-mail: farrah.deeba@uaf.edu.pk and tariq@stu.njau.edu.cn

DOI: 10.14202/vetworld.2025.1741-1747

Copyright: Adil, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.