Vet World Vol.18 March-2025 Article - 7
Research Article
Veterinary World, 18(3): 598-605
https://doi.org/10.14202/vetworld.2025.598-605
Comparison of DL-Methionine and L-Methionine levels in liver metabolism activity in commercial broilers fed a diet without antibiotic growth promoters
2. PT. Cheil Jedang Indonesia, South Jakarta, Jakarta 12710, Indonesia.
3. Division of Pathology, School of Veterinary Medicine and Biomedical Sciences, IPB University, West Java16680, Indonesia.
4. eLRosa Laboratory, iRATco Group, 16680 West Java, Indonesia.
5. Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, West Java16680, Indonesia.
Background and Aim: Methionine is an essential amino acid in broiler nutrition, playing a crucial role in growth performance and liver metabolism. As an alternative to antibiotic growth promoters (AGPs), this study aimed to evaluate the effects of DL-Methionine and L-Methionine supplementation on liver metabolism, antioxidant activity, and growth performance in broilers raised without AGPs.
Materials and Methods: A total of 440 one-day-old Cobb 500 male broiler chicks were assigned to 11 groups: A control group and 10 treatment groups receiving graded levels (70%, 85%, 100%, 115%, and 130%) of either DL-Methionine or L-Methionine. The study assessed body weight, liver mass index, D-amino acid oxidase (DAAO) activity, serum glutathione concentration, and liver histopathology across three growth stages: Starter (day 11), grower (day 25), and finisher (day 35).
Results: L-Methionine supplementation resulted in greater body weight gain compared to DL-Methionine, particularly in the finisher stage. DL-Methionine demonstrated a stronger influence on liver metabolism by increasing DAAO activity and reducing oxidative stress, as indicated by lower serum glutathione levels. No significant histopathological alterations were observed among the groups, confirming the safety of both methionine sources.
Conclusion: While L-Methionine improved growth performance, DL-Methionine effectively enhanced liver metabolism and reduced oxidative stress. These findings suggest that DL-Methionine may support liver function, whereas L-Methionine is more effective for weight gain in broilers raised without AGPs.
Keywords: antioxidant activity, broiler chickens, DL-Methionine, liver metabolism, L-Methionine.
How to cite this article: Andriyanto A, Sanoesi TBT, Putra AA, Subangkit M, Fadholly A, Putra HY, and Rotinsulu DA (2025) Comparison of DL-Methionine and L-methionine levels in liver metabolism activity in commercial broilers fed a diet without antibiotic growth promoters, Veterinary World, 18(3): 598-605.
Received: 12-09-2024 Accepted: 04-02-2025 Published online: 18-03-2025
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2025.598-605
Copyright: Andriyanto, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
