Vet World Vol.10 August-2017 Article-22
Research Article
Veterinary World, 10(8): 964-972
https://doi.org/10.14202/vetworld.2017.964-972
Molecular etiopathology of naturally occurring reproductive diseases in female goats
2. Centre for Animal Disease Research and Diagnosis (CADRAD), ICAR-Indian Veterinary Research Institute, Izatnagar - 243 122, Uttar Pradesh, India.
3. Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar - 243 122, Uttar Pradesh, India.
Background and Aim: The aim of the present study was to investigate the molecular etiopathology of occurrence of reproductive diseases in female goats. Reproductive diseases in goats account for major economic losses to goat farmers in terms of valuable loss of offspring and animal productivity.
Materials and Methods: A total of 660 female genitalia were examined for pathological conditions (macroscopic and microscopic lesions). The etiopathological study was carried out for the presence of pathogenic organisms such as Brucella, Chlamydia, and Campylobacter in the uterus and ovary. Based on the microscopic lesions, suspected samples were subjected to diagnostic polymerase chain reaction (PCR) for various etiological agents employing 16srRNA genus specific primers for Campylobacter and Chlamydophila and OMP31 gene-based PCR for Brucella melitensis and nested PCR using ITS-1 gene primers for Toxoplasma gondii. For Brucella suspected samples, immunohistochemistry (IHC) was also performed.
Results: In studied female genitalia, 108 (16.30%) showed gross abnormalities with overall 23.32% occurrence of pathological conditions (macroscopic and microscopic lesions). Pathological involvement of the uterus was the highest 68 (62.96%), followed by the ovaries 27 (25%) and other organs. Major uterine condition observed was endometritis (5.60%). In uterine infections, 35 (5.30%) samples were found positive for Campylobacter spp., 12 (1.81%) samples for B. melitensis, and 3 (0.45%) samples were positive for Chlamydophila spp. Among the samples positive for B. melitensis by PCR, 3 were found positive by IHC also. Corynebacterium ovis was detected by PCR using specific primers in a case of hydrosalpinx. It was concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.
Conclusion: The present study concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats. Keywords: Brucella melitensis, Campylobacter spp., Chlamydophila spp., Corynebacterium ovis, etiopathology, female genitalia, goat, ovary, reproductive diseases, uterus.
Keywords: Brucella melitensis, Campylobacter spp., Chlamydophila spp., Corynebacterium ovis, etiopathology, female genitalia, goat, ovary, reproductive diseases, uterus.
How to cite this article: Beena V, Pawaiya RVS, Gururaj K, Singh DD, Mishra AK, Gangwar NK, Gupta VK, Singh R, Sharma AK, Karikalan M, Kumar A (2017) Molecular etiopathology of naturally occurring reproductive diseases in female goats, Veterinary World, 10(8): 964-972.
Received: 10-03-2017 Accepted: 26-07-2017 Published online: 22-08-2017
Corresponding author: R. V. S. Pawaiya E-mail: rvspawaiya@gmail.com
DOI: 10.14202/vetworld.2017.964-972
Copyright: Beena, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.