Vet World Vol.11 January-2018 Article-6
Research Article
Veterinary World, 11(1): 25-29
https://doi.org/10.14202/vetworld.2018.25-29
Anti-inflammatory properties of a wound dressing combination of zinc oxide and turmeric extract
2. Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
3. Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
4. Dentist, Surabaya, Indonesia.
Background and Aim: The aim of this study was to determine the effect of a wound dressing consisting of a zinc oxide with turmeric extract combination as an anti-inflammatory on the healing process through the expression of MAC387 and cyclooxygenase-2 (COX-2).
Materials and Methods: Forty Wistar rats were divided into four control and four treatment groups (n=5). On day 1, a 6 mmx6 mm square of skin in the area of the vertebralis thoracis was excised from all subjects. The wound was then dressed with a combination of zinc oxide and turmeric extract for the treatment groups, while the control groups were left undressed. Both the control and treatment groups were then sequentially sacrificed on days 3, 5, 7, and 14 to obtain subepithelial excision samples. These samples subsequently underwent immunohistochemistry examination through the expression of MAC387 and COX-2 to ascertain the anti-inflammatory reaction to the wound healing process.
Results: The highest expression of MAC387 was found in the treatment group to which a dressing of zinc oxide with turmeric extract had been applied on the day 5 before slowly reducing on days 7 and 14. MAC387 peaked in the undressed control group on day 14. The COX-2 expression results in control groups showed their higher expression on day 3, increased up to day 5, began to decline on day 7 before, and finally, decreasing on day 14. This result was different to those treatment groups which presented a high COX-2 expression on day 3, before gradually decreasing between days 5 and 7 and reaching its lowest point on day 14.
Conclusion: A wound dressing consisting of a combination of zinc oxide and turmeric extract has been proven effective as an anti-inflammatory in the healing process. Keywords: cyclooxygenase-2, MAC387, turmeric, wound dressing, zinc oxide.
Keywords: cyclooxygenase-2, MAC387, turmeric, wound dressing, zinc oxide.
How to cite this article: Meizarini A, Aryati, Riawan W, Puteri A (2018) Anti-inflammatory properties of a wound dressing combination of zinc oxide and turmeric extract, Veterinary World, 11(1): 25-29.
Received: 13-10-2017 Accepted: 06-12-2017 Published online: 17-01-2018
Corresponding author: Asti Meizarini E-mail: asti-m@fkg.unair.ac.id
DOI: 10.14202/vetworld.2018.25-29
Copyright: Meizarini, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.