Effects of intratesticular injection of zinc-based solution in rats in combination with anti-inflammatory and analgesic drugs during chemical sterilization

Vet World Vol.11 May-2018 Article-12

Research Article

Veterinary World, 11(5): 649-656

https://doi.org/10.14202/vetworld.2018.649-656

Simone Regina Barros de Macedo¹, Luiz Andre Rodrigues de Lima¹, Sandra Maria de Torres¹, Vinicius Vasconcelos Gomes de Oliveira¹, Rosana Nogueira de Morais², Christina Alves Peixoto³, Bruno Mendes Tenorio⁴, and Valdemiro Amaro da Silva Junior¹

1. Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil.
2. Department of Physiology, Federal University of Parana, Curitiba, Parana, Brazil.
3. Laboratory of Ultrastructure, Aggeu Magalhaes Research Center (CPqAM), Recife, Pernambuco, Brazil.
4. Department of Morphology, Federal University of Paraiba, Joao Pessoa, Paraiba, Brazil.

Background and Aim: Chemical sterilization is a non-surgical method of contraception based on compounds injected into the testis to induce infertility. However, these injections can cause discomfort and pain able to impair the recovery of animals after this treatment. The objective of this study was to investigate if anti-inflammatories or pain relievers inhibited the sterilizing effect of zinc gluconate-based solution on the testis.

Materials and Methods: Adult rats were treated in groups: G1 (control), G2 (dimethyl sulfoxide + dipyrone); G3 (dipyrone/ zinc); G4 (dipyrone + celecoxib/zinc); G5 (dipyrone + meloxicam/zinc), and G6 (dipyrone + dexamethasone/zinc) in a single dose per day during 7 days. Animals were analyzed at 7, 15, and 30 days after treatments.

Results: The zinc-induced a widespread testicular degeneration and decreased testosterone levels even in combination with anti-inflammatories or pain relievers. Testis, epididymis, prostate, and seminal vesicle had a weight reduction. The anti-inflammatory effect of dexamethasone interfered in the desired action of zinc gluconate in the 1st 15 days and celecoxib up to 7 days.

Conclusion: Meloxicam plus dipyrone did not impair the chemical sterilization based on zinc gluconate, and it can be used to reduce nociceptive effects in animals after chemical sterilization. Keywords: analgesic, anti-inflammatory, contraception, testicular degeneration, zinc gluconate.

Keywords: analgesic, anti-inflammatory, contraception, testicular degeneration, zinc gluconate.

How to cite this article: de Macedo SRB, de Lima LAR, de Torres SM, de Oliveira VVG, de Morais RN, Peixoto CA, Tenorio BM, da Silva Junior VA (2018) Effects of intratesticular injection of zinc-based solution in rats in combination with anti-inflammatory and analgesic drugs during chemical sterilization, Veterinary World, 11(5): 649-656.

Received: 15-02-2018 Accepted: 16-04-2018 Published online: 19-05-2018

Corresponding author: Valdemiro Amaro da Silva Junior E-mail: valdemiro.asilvajr@ufrpe.br

DOI: 10.14202/vetworld.2018.649-656

Copyright: de Macedo SRB, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.