In vitro assessment of antibacterial activity from Lactobacillus spp. strains against virulent Salmonella species isolated from slaughter animals in Benin

Vet World Vol.12 December-2019 Article-10

Research Article

Veterinary World, 12(12): 1951-1958

https://doi.org/10.14202/vetworld.2019.1951-1958

Alidehou Jerrold Agbankpe¹, Tamegnon Victorien Dougnon¹, Roubaya Balarabe¹, Esther Deguenon¹, and Lamine Baba-Moussa²

1. Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
2. Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin.

Background and Aim: Salmonella spp. are among the world's leading foodborne pathogens, found naturally in the intestines of many animals. Lactic acid bacteria, mainly Lactobacillus, are a promising alternative to antibiotics for animal and human health. This study aimed to assess the in vitro antibacterial activity of Lactobacillus spp. strains against virulent Salmonella spp. isolated from slaughter animals in Benin.

Materials and Methods: Eleven samples of raw cow's milk, five samples of breast milk, and six infant stool samples were taken. From these samples, strains of Lactobacillus were isolated and identified. The probiotic potential of each of the identified strains was characterized, and finally in vitro antibacterial activity of these strains was evaluated against three virulent strains of Salmonella spp. and a reference strain of Salmonella Typhimurium ATCC 14028.

Results: Out of the 22 samples collected, 20 strains of Lactobacillus spp. were isolated and identified. These strains included Lactobacillus plantarum (30%), Lactobacillus delbrueckii (25%), Lactobacillus casei (25%), Lactobacillus salivarius (15%), and Lactobacillus acidophilus (05%). Characterization of the probiotic potential of these strains showed that only 16 strains were resistant to pH=1.5. Fourteen of them were able to withstand the simulated gastric juice (pH 1.5+pepsin). The 14 probiotic strains showed very good antibacterial activity against virulent strains of Salmonella spp. with inhibition zone diameters ranging from 12.36±0.03 mm to 35.33±0.05 mm (R values>6 mm).

Conclusion: From this study, Lactobacillus strains isolated from raw cow milk, breast milk, and infantile stool might be used as some valid candidates for probiotics. It also represents good alternatives for antibiotics in the fight against animal and human salmonellosis. Keywords: antibacterial activity, Benin, Lactobacillus spp., probiotic, Salmonella spp.

Keywords: antibacterial activity, Benin, Lactobacillus spp., probiotic, Salmonella spp.

How to cite this article: Agbankpe AJ, Dougnon TV, Balarabe R, Deguenon E, Baba-Moussa L (2019)

Received: 31-07-2019 Accepted: 08-11-2019 Published online: 13-12-2019

Corresponding author: Alidehou Jerrold Agbankpe E-mail: agbankpejerrold@yahoo.fr

DOI: 10.14202/vetworld.2019.1951-1958

Copyright: Agbankpe, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.