Effects of bone types, particle sizes, and gamma irradiation doses in feline demineralized freeze-dried bone allograft

Vet World Vol.13 August-2020 Article-7

Research Article

Veterinary World, 13(8): 1536-1543

https://doi.org/10.14202/vetworld.2020.1536-1543

Frizky Amelia^1,2, Basril Abbas³, Darmawan Darwis³, Sri Estuningsih⁴, and Deni Noviana^2,4

1. Program Study of Animal Biomedical Science, Graduate School of IPB University, Bogor, Jawa Barat 16680, Indonesia.
2. Diagnostic Imaging Center, Veterinary Teaching Hospital, Faculty of Veterinary Medicine IPB University, Bogor, Jawa Barat 16680, Indonesia.
3. Centre for Isotopes and Radiation Application, National Nuclear Energy Agency (BATAN), Jakarta Selatan, DKI Jakarta 12440, Indonesia.
4. Department of Clinic Reproduction and Pathology, Faculty of Veterinary Medicine IPB University, Bogor, Jawa Barat 16680, Indonesia.

Background and Aim: Fracture cases significantly increase recently, demanding high quality of bone graft materials. This research aimed to evaluate the effects of bone types, particle sizes, and gamma irradiation doses on morphological performance and cell viability of feline demineralized freeze-dried bone allograft (DFDBA) through an in vitro study.

Materials and Methods: Feline DFDBA derived from feline cortical and cancellous long bones was processed into four different sizes: Group A (larger than 1000 μm), B (841-1000 μm), C (420-840 μm), and D (250-419 μm) for each type of bones. The materials were then irradiated with two doses of gamma rays, 15 and 25 kGy, resulting in 16 variants of feline DFDBA. The surfaces of each material were then observed with the scanning electron microscope (SEM). The in vitro evaluation of feline DFDBA was then performed using 3-(4,5-dimethythiazol-2)-2,5-diphenyltetrazolium bromide (MTT) assay with calf pulmonary artery endothelial cells.

Results: The MTT assay results showed that the lowest inhibition rate (14.67±9.17 %) achieved by feline DFDBA in Group A derived from cortical bones irradiated with 15 kGy. Group D generally showed high inhibition rate in both cancellous and cortical bones, irradiated with either 15 or 25 kGy. The SEM results showed that cancellous and cortical bones have numerous macropores and micropores structure in 170× and 3000×, respectively.

Conclusion: The material derived from cortical bones in Group A (larger than 1000 μm in particle size) irradiated with 15 kGy is the best candidate for further development due to its abundance of micropores structure and ability in preserving the living cells. Keywords: bone type, feline bone allograft, gamma irradiation, in vitro study, particle size.

Keywords: bone type, feline bone allograft, gamma irradiation, in vitro study, particle size.

How to cite this article: Amelia F, Abbas B, Darwis D, Estuningsih S, Noviana D (2020) Effects of bone types, particle sizes, and gamma irradiation doses in feline demineralized freeze-dried bone allograft, Veterinary World, 13(8): 1536-1543.

Received: 13-01-2020 Accepted: 09-06-2020 Published online: 10-08-2020

Corresponding author: Deni Noviana E-mail: deni@apps.ipb.ac.id

DOI: 10.14202/vetworld.2020.1536-1543

Copyright: Amelia, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.