Vet World Vol.13 May-2020 Article-22
Research Article
Veterinary World, 13(5): 981-986
https://doi.org/10.14202/vetworld.2020.981-986
Molecular characterization and phylogenetic analysis of fowl adenovirus serotype-4 from Guangdong Province, China
2. Guangdong Women and Children Hospital, Guangdong Province, China.
3. Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr el-Sheikh 33516, Egypt.
Background and Aim: Our aim in this study was to isolate potentially novel strains of fowl adenovirus serotype-4 (FAdV-4) that is currently circulating in broiler chicken flocks in Guangdong Province, China, and to compare nucleotide and amino acid (AA) sequences of their respective hexon genes.
Materials and Methods: The experiment was carried out on poultry farms experiencing outbreaks of FAdV-4-associated hydropericardium syndrome (HPS). Tissue samples from the hearts and livers of deceased chickens were screened for FAdV-4 infection using hexon gene-specific polymerase chain reaction (PCR).
Results: New virus isolates were used to infect 7-day-old chicks, which went onto reproduce typical HPS signs. The hypervariable region of the FAdV-4 hexon gene was PCR-amplified and sequenced. The hexon nucleotide and deduced AA sequence identities were 99.8-99.9% and 99.5-99.8%, respectively, among the four novel isolates. In addition, the new isolates were 97-100% and 96.4-99.9% identical to the nucleotide and deduced AA sequences, respectively, of FAdV-4 hexon genes available in the National Center for Biotechnology Information GenBank database. Phylogenetic analyses, based on the hexon gene sequence, revealed that the new isolates, clustered with FAdV-C; the FAdV-A, FAdV-B, FAdV-D, and FAdV-E viruses, were more distantly related.
Conclusion: New FAdV-4 isolates from Guangdong Province are similar to those identified in other regions of the world. This information provides critical insight into HPS epidemiology and provides a perspective for monitoring outbreaks and developing strategies for disease prevention. Keywords: Angara disease, fowl adenovirus-4, phylogenetic analysis.
Keywords: Angara disease, fowl adenovirus-4, phylogenetic analysis.
How to cite this article: Yuming F, Sheng Y, Wenyu D, Shihong C, Wenfeng L, Wenjing H, Xiaowen L, El-Ashram S, Mei K, Jinyue G, Xuelian Z, Zhili L, Shujian H (2020) Molecular characterization and phylogenetic analysis of fowl adenovirus serotype-4 from Guangdong Province, China, Veterinary World, 13(5):981-986.
Received: 26-11-2019 Accepted: 09-04-2020 Published online: 24-05-2020
Corresponding author: Huang Shujian E-mail: huangshujian@fosu.edu.cn
DOI: 10.14202/vetworld.2020.981-986
Copyright: Yuming, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.