Vet World Vol.15 May-2022 Article-22
Research Article
Veterinary World, 15(5): 1323-1327
https://doi.org/10.14202/vetworld.2022.1323-1327
First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq
2. Unit of Zoonotic Disease Research, College of Veterinary Medicine, University of Al-Qadisiyah, Al Diwaniyah, Iraq.
Background and Aim: Hemotropic Mycoplasmas are small epierythrocytic bacteria that cause infectious anemia in several livestock species and in humans. Several reports have been made on hemoplasma infections in the south and north of Iraq, but there have been no studies in the middle Euphrates of Iraq. This study aimed to evaluate the presence of hemoplasma species in cattle in Al-Qadisiyah Province, Iraq.
Materials and Methods: Two hundred blood samples were collected from cattle with pale mucous membrane from regions with heavy tick endemicity. The samples were analyzed for the presence of Rickettsia pathogens using thin blood smears and the Diff-Quik stains. All the samples were also examined using polymerase chain reaction (PCR) to amplify the 16S ribosomal ribonucleic acid (rRNA) gene to confirm the presence of the smear-identified microorganisms. Ten PCR positive samples were subjected to 16S rRNA partial gene sequencing to identify the species.
Results: The findings uncovered positivity in 68 (34%) blood smears. PCR revealed positive confirmation in 18 (9%) of the 200 blood samples. Mycoplasma wenyonii and Candidatus mycoplasma hemobos were identified from 10 PCR positive samples. The nucleotide sequences of the isolates were closely related to isolates from cattle, buffalo, and dogs in Vietnam, Cuba, India, and Germany.
Conclusion: Bovine hemoplasma infections are prevalent in cattle in the Al-Qadisiyah Province in Iraq. Our results may have significance for the development of control programs. Keywords: hemoplasma spp., microscopic, molecular diagnosis, Mycoplasma wenyonii, Iraq.
Keywords: hemoplasma spp., microscopic, molecular diagnosis, Mycoplasma wenyonii, Iraq.
How to cite this article: Khudhair YI, Saleh ZF, Ayyez HN (2022) First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq, Veterinary World, 15(5): 1323-1327.
Received: 17-11-2021 Accepted: 25-03-2022 Published online: 26-05-2022
Corresponding author: Yahia Ismail Khudhair E-mail: yahia.khudiar@qu.edu.iq
DOI: 10.14202/vetworld.2022.1323-1327
Copyright: Khudhair, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.