Vet World Vol.16 March-2023 Article-21
Research Article
Veterinary World, 16(3): 595-600
https://doi.org/10.14202/vetworld.2023.595-600
Psychological dominant stressor modification to an animal model of depression with chronic unpredictable mild stress
2. Department of Child Health, Faculty of Medicine, Widya Mandala Catholic University, Surabaya, Indonesia.
3. Department of Child Health, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
4. Department of Psychiatry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Background and Aim: Chronic unpredictable mild stress (CUMS) is a protocol widely used to create an animal model of depression with food deprivation, water deprivation, and physical-dominant stressors as routine procedures. However, human depression mainly involves psychological stressors and does not always involve a lack of food and water; thus, CUMS procedures should be modified accordingly. Therefore, this study aimed to create an animal model of depression, mainly focusing on a psychologically dominant stressor without food and water deprivation.
Materials and Methods: The CUMS and control groups, respectively, received CUMS modification (psychologically dominant stressors without food and water deprivation) for 21 days. A 24-h sucrose preference test (SPT) was used to assess the successful creation of an animal model of depression. Daily food intake measurements, weekly weight monitoring, and weight gain calculations were performed. Either an independent sample t-test or the Mann–Whitney test was used.
Results: Of the 42 rats included, 39 completed the study. Chronic unpredictable mild stress procedures for 21 days significantly reduced the SPT (p < 0.05), mean body weight (p < 0.05), and weekly weight gain (p < 0.05) in the CUMS group compared to the control group. However, the weekly average food intake did not statistically differ between the two groups.
Conclusion: Psychological dominant CUMS modification to an animal model of depression resulted in lower SPT, body weight, and weekly weight gain in the CUMS group than in the control group. Keywords: body weight, chronic unpredictable mild stress, modification, psychological, rat, sucrose preference test.
Keywords: body weight, chronic unpredictable mild stress, modification, psychological, rat, sucrose preference test.
How to cite this article: Pangemanan L, Irwanto I, and Maramis MM (2023) Psychological dominant stressor modification to an animal model of depression with chronic unpredictable mild stress, Veterinary World, 16(3): 595-600.
Received: 10-12-2022 Accepted: 09-02-2023 Published online: 24-03-2023
Corresponding author: Irwanto Irwanto E-mail: irwanto@fk.unair.ac.id
DOI: 10.14202/vetworld.2023.595-600
Copyright: Pangemanan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.