Vet World Vol.17 June-2024 Article - 12
Research Article
Veterinary World, 17(6): 1281-1290
https://doi.org/10.14202/vetworld.2024.1281-1290
Study of different heterocycles showing significant anti-severe acute respiratory syndrome 2 activity in vitro and in vivo
2. Department of Chemistry Laboratory of Structural Bioinformatics, Russian-Armenian University, H. Emin, 123, Yerevan, 0051, Armenia.
3. Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia.
4. Department of Mathematics, Institute for Informatics and Automation Problems of NAS RA, Yerevan, Armenia.
5. Department of Anatomy, Yerevan State Medical University after M. Heratsi, Armenia Yerevan, Armenia.
6. United Institute of Informatics Problems, National Academy of Sciences of Belarus, Belarus.
7. Department of Epidemiology and Parasitology, Armenian National Agrarian University, Yerevan, Armenia.
8. Laboratory of Cell Biology and Virology, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia.
9. Department of Human Anatomy, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.
Background and Aim: With the emergence of severe acute respiratory syndrome-related coronavirus (SARS-CoV-2), antiviral drug development has gained increased significance due to the high incidence and potentially severe complications of the resulting coronavirus infection. Heterocycle compounds, acting as antimetabolites of DNA and RNA monomers, rank among the most effective antiviral drugs. These compounds’ antiviral effects on various SARS-CoV-2 isolates, as found in existing data collections, form the basis for further research. The aim of this study was to examine the possible antiviral effect of some originally synthesized heterocyclic compounds.
Materials and Methods: The main methods were cell culturing, cytotoxicity assay, qRT-PCR assay, tissue and blood cells analysis, and micro-computed tomography (micro-CT) imaging.
Results: In both in vitro and in vivo conditions, the elimination of SARS-Cov-2 occurred significantly earlier after administration of the compounds compared to the control group. In hamsters, the primary symptoms of coronavirus disease disappeared following administration of heterocycle compounds.
Conclusion: Using delta and omicron strains of the SARS-CoV-2 virus, newly created heterocycle compound analogs dramatically reduced SARS-CoV-2 multiplication, resulting in a drop in viral RNA load in the supernatant under in vitro conditions. Improvements in pathological manifestations in the blood, bone marrow, and internal organs of hamsters demonstrated that heterocycle compounds inhibited SARS-CoV-2 replication both in vitro and in vivo.
Keywords: broad-spectrum antiviral agents, heterocycle compounds, in vitro, in vivo, severe acute respiratory syndrome-related coronavirus, Syrian hamsters.
How to cite this article: Yengoyan A, Gomktsyan T, Pivazyan V, Ghazaryan E, Shainova R, Karapetyan A, Avetyan D, Aslanyan L, Baroyan K, Tuzikov A, Sargsyan M, Baghdasaryan B, Bayramyan N, Hakobyan S, Poghosyan A, Avetisyan A, Avagyan H, Hakobyan L, and Karalyan Z (2024) Different heterocycles show significant anti-severe acute respiratory syndrome 2 activity in vitro and in vivo, Veterinary World, 17(6): 1281-1290.
Received: 2024-02-22 Accepted: 2024-05-13 Published online: 2024-06-14
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.1281-1290
Copyright: Yengoyan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
