Evaluation of the effects of green tea extract as a dietary supplement in sheep on gas production, volatile fatty acids, and digestibility

Vet World Vol.17 October-2024 Article - 2

Research Article

Veterinary World, 17(10): 2204-2210

https://doi.org/10.14202/vetworld.2024.2204-2210

Ririn Siti Rahmatillah¹, Diky Ramdani¹, Iman Hernaman², Anuraga Jayanegara³, and Nanik Hidayatik⁴

1. Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, West Java, 45363, Indonesia.
2. Department of Animal Nutrition and Feed Technology, Faculty of Animal Husbandry, Universitas Padjadjaran, Jatinangor Campus, Sumedang 45363, Indonesia.
3. Department of Animal Nutrition and Feed Technology, Faculty of Animal Husbandry, IPB University, Bogor, 16680 Indonesia.
4. Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60132 Indonesia.

Background and Aim: Phenolic tannins, which are ubiquitous in plants, exhibit diverse biological activities and have drawn significant attention for their potential impact on ruminant nutrition and health. Although phenolic tannins have beneficial and detrimental effects on rumen fermentation, their precise influence remains poorly understood. This study aimed to explore the effects of varying doses of green tea extract (GTE) on rumen fermentation parameters through an in vitro trial using sheep rumen liquids.

Materials and Methods: A 4 × 2 factorial design was used to test the effect of 4 different doses of GTE treatment (0, 140, 280, and 560 mg/kg) in 2 different in vitro runs on degradability, fermentation profiles, and gas production using the in vitro Hohenheim Gas Test method.

Results: Across running times, the GTE-treated diet did not affect (p > 0.05) dry matter degradability % and organic matter degradability %, pH, ammonia (NH3-N, mg/dL), 24 h total gas production (tGP 24h, mL), and acetate-to-propionate ratio (A: P), but it reduced (p < 0.05) tGP 6 h compared with GTE-0 (control diet without GTE). GTE treatment tended to reduce (p < 0.1) methane (CH4, % LEL) and total volatile Fatty Acids (tVFA, mM). Across GTE treatment, the 1st in vitro run had higher (p < 0.001) tGP 6–24 h and pH, but lower (p < 0.001) tVFA and A: P in comparison with the 2^nd in vitro run.

Conclusion: GTE treatment tends to decrease CH4 output in rumen without affecting degradability, tGP, and most fermentation profiles, except for a tendency to reduce tVFA.

Keywords: Camellia sinensis, Garut sheep, green tea extract, in vitro.

How to cite this article: Rahmatillah RS, Ramdani D, Hernaman I, Jayanegara A, and Hidayatik N (2024) Evaluation of the effects of green tea extract as a dietary supplement in sheep on gas production, volatile fatty acids, and digestibility, Veterinary World, 17(10): 2204-2210.

Received: 2024-04-16 Accepted: 2024-09-03 Published online: 2024-10-04

Corresponding author: Diky Ramdani E-mail: diky.ramdani@unpad.ac.id

DOI: 10.14202/vetworld.2024.2204-2210

Copyright: Rahmatillah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.