Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics

Vet World Vol.18 January-2025 Article - 12

Research Article

Veterinary World, 18(1): 102-109

https://doi.org/10.14202/vetworld.2025.102-109

Athhar Manabi Diansyah^1,2, Sanstoso Santoso¹, Herdis Herdis¹, Muhammad Yusuf², Tri Puji Priyatno¹, Tulus Maulana³, Abdul Latief Toleng², Muhammad Ihsan Andi Dagong², Syahruddin Said³, Hikmayani Iskandar¹, Aeni Nurlatifah^1,4, Puji Lestari¹, Lukman Affandy¹, and Abdullah Baharun⁵

1. Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.
2. Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Indonesia. Jl. Perintis Kemerdekaan 10 Tamalanrea Makassar, South Sulawesi 90245, Indonesia.
3. Research Center for Applied Zoology, National Research and Innovation Agency, Jl. Raya Bogor Km. 46, Cibinong, West Java 16911, Indonesia.
4. Department of Nutrition and Feed Technology, Faculty of Animal Science, Gadjah Mada University, Depok, Sleman Regency, Special Region of Yogyakarta, Indonesia, 55281.
5. Department of Animal Science, Faculty of Agriculture, Djuanda University, Jl. Tol Ciawi No. 1, Ciawi, West Java 16720, Indonesia.

Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls.

Materials and Methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography–tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions.

Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction.

Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.

Keywords: artificial insemination, Bali-polled bulls, fertility biomarkers, seminal plasma proteomics, sperm kinematics.

How to cite this article: Diansyah AM, Santoso S, Herdis H, Yusuf M, Priyatno TP, Maulana T, Toleng AL, Dagong MIA, Said S, Iskandar H, Nurlatifah A, Lestari P, Affandy L, and Baharun A (2025) Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics, Veterinary World, 18(1): 102-109.

Received: 2024-06-24 Accepted: 2024-11-25 Published online: 2025-01-14

Corresponding author: Athhar Manabi Diansyah E-mail: athhar.md@unhas.ac.id

DOI: 10.14202/vetworld.2025.102-109

Copyright: Diansyah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.