Vet World   Vol.18   November-2025  Article - 19 

Optimization of insulin-like growth factor-1 supplementation enhances oocyte quality by modulating oxidative stress and apoptotic pathways during in vitro maturation of Kacang goat oocytes

Widjiati Widjiati¹ , Epy Muhammad Luqman¹ , Ninik Darsini² , Aulanni’am Aulanni’am³ , Wan Nor Fitri Bin Wan Jaafar⁴ , Suzanita Utama⁵ , Devia Yoanita Kurniawati¹ , Zahra Shabira¹ , and Viski Fitri Hendrawan⁶

Background and Aim: Insulin-like growth factor-1 (IGF-1) plays a crucial role in folliculogenesis and oocyte maturation by regulating oxidative stress and apoptotic pathways. However, the optimal IGF-1 concentration for small ruminant oocytes, particularly the native Kacang goat, remains undefined. This study aimed to determine the optimal IGF-1 dose for improving oocyte quality during in vitro oocyte maturation (IVM) by evaluating oxidative stress and apoptosis markers.

Materials and Methods: Ovaries (n = 120) were collected from local slaughterhouses, and cumulus–oocyte complexes were aspirated, selected, and randomly divided into four groups: Control (0 ng/mL IGF-1) and treatments with 50, 100, and 150 ng/mL IGF-1 supplementation. Mature oocytes were analyzed for oxidative stress biomarkers, including superoxide dismutase (SOD-1), glutathione (GSH), and malondialdehyde (MDA), using an enzyme-linked immunosorbent assay, and for apoptosis regulators, B-cell lymphoma 2-associated X protein (BAX), B-cell lymphoma-2 (BCL-2), and cytochrome c, using immunocytochemistry. Data were analyzed using one-way analysis of variance and Duncan’s post hoc test (p < 0.05).

Results: IGF-1 supplementation produced concentration-dependent effects. The 100 ng/mL group (T2) exhibited the highest SOD-1 (2.07 ± 0.60) and GSH (8.07 ± 1.79) levels (p < 0.05), while MDA increased with higher IGF-1 doses, indicating a threshold beyond which oxidative stress is induced. Anti-apoptotic BCL-2 and cytochrome c expressions peaked at 50 ng/mL IGF-1 (10.73 ± 1.56 and 11.73 ± 0.99, respectively), whereas the pro-apoptotic marker BAX was lowest in the same group and increased at higher doses. The findings suggest that 50 ng/mL IGF-1 effectively maintains redox balance and mitochondrial stability through phosphatidylinositol 3-kinase/protein kinase B-mediated regulation.

Conclusion: This study demonstrates, for the 1st time, a dose-dependent, biphasic effect of IGF-1 on oxidative and apoptotic pathways in Kacang goat oocytes. An optimal concentration of 50 ng/mL IGF-1 enhances oocyte maturation by balancing antioxidant defense and anti-apoptotic mechanisms, whereas higher concentrations induce oxidative stress. These findings establish a breed-specific IVM optimization protocol that supports genetic preservation and sustainable reproductive biotechnology for indigenous goats.

Keywords: apoptosis, in vitro oocyte maturation, Insulin-like growth factor-1, Kacang goat, oocyte quality, oxidative stress, phosphatidylinositol 3-kinase/protein kinase B.

How to cite this article: Widjiati W, Luqman EM, Darsini N, Aulanni’am A, Jaafar WNFBW, Utama S, Kurniawati DY, Shabira Z, and Hendrawan VF (2025) Optimization of insulin-like growth factor-1 supplementation enhances oocyte quality by modulating oxidative stress and apoptotic pathways during in vitro maturation of Kacang goat oocytes, Veterinary World, 18(11): 3545-3560.

Received: 07-07-2025   Accepted: 24-10-2025   Published online: 27-11-2025

Corresponding author: Widjiati Widjiati    E-mail: widjiati@fkh.unair.ac.id

DOI: 10.14202/vetworld.2025.3545-3560

Copyright: Widjiati, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.