Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Research (Published online: 29-11-2018)

18. Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Gusti Ayu Yuniati Kencana, Nyoman Suartha, I Made Kardena, Gusti Ayu Mayani Kristina Dewi, Arini Nurhandayani, Syamsidar and Kadek Karang Agustina

Veterinary World, 11(11): 1637-1640

Gusti Ayu Yuniati Kencana: Department of Virology, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.

Nyoman Suartha: Department of Internal Medicine, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.

I Made Kardena: Department of Pathology, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.

Gusti Ayu Mayani Kristina Dewi: Department of Poultry Science, Faculty Animal Husbandry, Udayana University, Denpasar, Indonesia.

Arini Nurhandayani: PT. Sanbio Laboratories, Bogor, Jawa Barat, Indonesia.

Syamsidar: PT. Sanbio Laboratories, Bogor, Jawa Barat, Indonesia.

Kadek Karang Agustina: Department of Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.

doi: 10.14202/vetworld.2018.1637-1640

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Article history: Received: 03-05-2018, Accepted: 11-10-2018, Published online: 29-11-2018

Corresponding author: Gusti Ayu Yuniati Kencana

E-mail: yuniati_kencana@unud.ac.id

Citation: Kencana GAY, Suartha N, Kardena IM, Dewi GAMK, Nurhandayani A, Syamsidar, Agustina KK (2018) Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia, Veterinary World, 11(11): 1637-1640.

Abstract

Aim: The study was aimed to prepare and examine the potential and safety concerns of egg drop syndrome (EDS) vaccine candidate seed. The potential and safety trials of EDS Medan isolate vaccine need to be done before commercial scale of EDS vaccines are made.

Materials and Methods: The safety test of EDS candidate vaccine was tested on 4-week-old specified pathogen-free chickens in an experimentally isolated enclosure.

Results: The result of the safety test obtained 2^7.3 hemagglutination inhibition (HI) unit of geometric mean titer antibody post-vaccination. However, the potency test of the EDS candidate vaccine was conducted on 17-week-old laying hens. Test results of the EDS potency vaccine in layer obtained antibody titer increased in every week of blood taking with average titer of antibody: Before vaccinated was 2^2.9 HI unit, 1 week after vaccination was 2^3.7 HI unit, 2 weeks post-vaccination was 2⁵ HI unit, and 3 weeks after vaccination was 2^7.3 HI units. In contrast, decreasing trend was observed in control group (unvaccinated chicken).

Conclusion: Serologically, the seed vaccine of EDS virus isolates from Medan was able to produce protective antibody titers starting in the 2nd and 3rd weeks post-vaccination.

Keywords: hemagglutination, inhibition test, Medan isolate, safety and potential test, seed of egg drop syndrome vaccine.

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