Vet World Vol.11 March-2018 Article-17
Research Article
Veterinary World, 11(3): 363-367
https://doi.org/10.14202/vetworld.2018.363-367
Detection of Brucella spp. in milk from seronegative cows by real-time polymerase chain reaction in the region of Batna, Algeria
2. Research Laboratory HASAQ, High National Veterinary School, Algiers, Algeria.
3. Laboratory of Molecular Biology and Microbiology of Constantine Biotechnology Research Center, Constantine, Algeria.
Background and Aim: The aim of this study was to detect Brucella spp. DNA in milk samples collected from seronegative cows using the real-time polymerase chain reaction (PCR) assay for diagnosis of brucellosis in seronegative dairy cows to prevent transmission of disease to humans and to reduce economic losses in animal production.
Materials and Methods: In this study, 65 milk samples were investigated for the detection of Brucella spp. The detection of the IS711 gene in all samples was done by real-time PCR assay by comparative cycle threshold method.
Results: The results show that of the 65 DNA samples tested, 2 (3.08%) were positive for Brucella infection. The mean cyclic threshold values of IS711 real-time PCR test were 37.97 and 40.48, indicating a positive reaction.
Conclusion: The results of the present study indicated that the real-time PCR appears to offer several advantages over serological tests. For this reason, the real-time PCR should be validated on representative numbers of Brucella-infected and free samples before being implemented in routine diagnosis in human and animal brucellosis for controlling this disease. Keywords: Brucella spp., milk, real-time polymerase chain reaction, seronegative cows.
Keywords: Brucella spp., milk, real-time polymerase chain reaction, seronegative cows.
How to cite this article: Sabrina R, Taha Mossadak H, Bakir M, Asma M, Khaoula B (2018) Detection of Brucella spp. in milk from seronegative cows by real-time polymerase chain reaction in the region of Batna, Algeria, Veterinary World, 11(3): 363-367.
Received: 03-12-2017 Accepted: 21-02-2018 Published online: 26-03-2018
Corresponding author: Mamache Bakir E-mail: mamachebakir@yahoo.fr
DOI: 10.14202/vetworld.2018.363-367
Copyright: Sabrina, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.