Vet World Vol.12 March-2019 Article-2
Research Article
Veterinary World, 12(3): 358-366
https://doi.org/10.14202/vetworld.2019.358-366
Determination of progesterone compounds in the crude methanol extract of benalu duku leaves
2. Department of Pharmacology, Faculty of Medical, Universitas Airlangga, Mayjen. Prof. Dr. Moestopo 47 Rd., "A" Campus Surabaya, Surabaya 60132, Indonesia.
3. Laboratory Histology, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Rd., "C" Campus Surabaya, Surabaya - 60115, Indonesia.
Background and Aim: Dendrophthoe pentandra L. Miq (benalu duku) is a parasitic herb that commonly grows on the host plant Lansium domesticum. Researchers have found that the plant contains anticancer compounds and may contain phytoandrogens, including progesterone-like compounds, in its crude methanol extract. The objective of the current study was to investigate the compound of phyto progesterone in benalu duku leaves after extracted by methanol and prepared using an analytical column of high-performance liquid chromatography (HPLC).
Materials and Methods: About 400 g of benalu duku leaves were pulverized, and their compounds were isolated by the isocratic method using an RP-18 analytical column (5 μm) with a mobile phase of 70:30 (methanol: water) in a photodiode array detector adjusted to 254 nm. The phyto progesterone compound was identified at a retention time of approximately 6.01 min.
Results: By LC-electrospray ionization mass spectrometry focusing on molecular fractions, the fingerprint area of the Fourier transform-infrared spectroscopy (FT-IR, cm−1) and Hnuclear magnetic resonance (NMR) spectra indicated that the phyto progesterone product isolated was identical to the certified reference material of pure progesterone, particularly the specific functional groups in the FT-IR spectrum at wavenumbers of 1317.43 cm−1 and 1386.86 cm−1 and in the proton HNMR spectrum at carbon 21 of progesterone (p<0.05).
Conclusion: Each 49.888 μg/mL of crude benalu duku leaf extract dissolved in the mobile phase contained 28.515±0.713 μg/mL phyto progesterone. Keywords: Androgenic phyto progesterone, Hnuclear magnetic resonance of crude methanol extract, Isolation technique of progesterone, molecular fractions, progesterone compounds in crude methanol extract, progesterone-like effect.
Keywords: Androgenic phyto progesterone, Hnuclear magnetic resonance of crude methanol extract, Isolation technique of progesterone, molecular fractions, progesterone compounds in crude methanol extract, progesterone-like effect.
How to cite this article: Mochamad L, Hermanto B, Hestianah EP (2019) Determination of progesterone compounds in the crude methanol extract of benalu duku leaves, Veterinary World, 12(3): 358-366.
Received: 29-10-2018 Accepted: 25-01-2019 Published online: 04-03-2019
Corresponding author: Lazuardi Mochamad E-mail: lazuardi@fkh.unair.ac.id
DOI: 10.14202/vetworld.2019.358-366
Copyright: Mochamad, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.