Vet World Vol.12 September-2019 Article-14
Research Article
Veterinary World, 12(9): 1448-1453
https://doi.org/10.14202/vetworld.2019.1448-1453
Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials
2. Department of Cardiology and Vascular Medicine , Dr. Soetomo General Hospital, Surabaya, Indonesia.
3. Department of Veterinary Clinical Science, Faculty of Veterinary, Universitas Airlangga, Surabaya, Indonesia.
4. PILAR Research and Education, Cambridge, UK.
Background and Aim: Several difficulties are involved in creating models for myocardial infarction (MI) in animals, such as low survival rates after acute MI, complicated techniques in creating animal models, complexities in confirming acute MI incidence, and complex surgical tools needed in the process. This study aimed to develop an animal model for acute MI using Wistar rats utilizing simple instruments that are readily available in standard animal laboratories.
Materials and Methods: We induced MI in 48 Wistar rats using the left anterior descending coronary artery ligation modification technique without tracheal incision and ventilator. This ligation technique was performed 1-2 mm distal to the left atrial appendage. MI occurrence was evaluated using heart enzyme parameters 24 h post-ligation and histological studies of the infarcted area 6 weeks after the ligation. Rats were divided into the coronary artery ligation group and sham group.
Results: Of the 48 rats, 24 (50%) died within 24 h post-ligation, but no further deaths occurred in the next follow-up period of 6 weeks. The average infarct size in six rats within 24 h of ligation was 35%±5.7%. The serum glutamic oxaloacetic transaminase level of the group treated with coronary artery ligation was statistically significantly higher than that of the sham group (p=0.000).
Conclusion: We developed an MI rat model with consistent infarction size, in which the long-term death of rats was not observed. Our ligation technique for an MI rat model can be a reference for experimental settings without ventilators for small animals. Keywords: left anterior descending coronary artery ligation, myocardial infarction, rat model.
Keywords: left anterior descending coronary artery ligation, myocardial infarction, rat model.
How to cite this article: Nugroho J, Yuniarti WM, Wardhana A, Ghea C (2019) Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials, Veterinary World, 12(9): 1448-1453.
Received: 29-05-2019 Accepted: 07-08-2019 Published online: 21-09-2019
Corresponding author: Johanes Nugroho E-mail: j.nugroho.eko@fk.unair.ac.id
DOI: 10.14202/vetworld.2019.1448-1453
Copyright: Nugroho, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.