Vet World Vol.14 December-2021 Article-1
Research Article
Veterinary World, 14(12): 3076-3083
https://doi.org/10.14202/vetworld.2021.3076-3083
Antioxidant activity and protective effect of propolis against carbon tetrachloride-induced liver and kidney injury by modulation of oxidative parameters
2. New York Medical Care for Nephrology, Richmond Hill, New York, United States.
3. Laboratory of Toxicology-Microbiology, and Environmental Health (UR11ES70), Faculty of Sciences of Sfax, Higher Institute of Biotechnology of Sfax, University of Sfax, Tunisia.
Background and Aim: Propolis has a protective effect against cellular damage caused by toxic agents such as drugs, metals, xenobiotics, and chemicals. The aim of this study was to investigate the antioxidant activity and the effect of ethanolic extract of propolis on carbon tetrachloride (CCl4)-induced oxidative stress on kidney and liver injury in rat.
Materials and Methods: The study quantified phenol, flavone, and flavonol in propolis and assessed antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and molybdate. The investigators used four groups of rats to study the effect of propolis on CCl4-induced toxicity. Propolis extract was given orally (500 mg/kg) for 12 days, and CCl4 (1 mL/kg) was administered intraperitoneally on day 5 of the experiment. Blood and tissue samples of the liver and kidney were collected on day 13 to measure biochemical and oxidative parameters. The parameters included malondialdehyde (MDA), protein carbonyl formation (PCO), advanced oxidation protein products (AOPP), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and ascorbic acid (AA). Biochemical parameters included liver enzymes, blood urea (BU), creatinine, and uric acid (UA).
Results: CCl4 decreased antioxidant agents, including CAT, GPx, GSH, and AA in the liver and kidney tissues. The oxidative agents' levels, including MDA, PCO, and AOPP, increased by CCl4 compared to the control group. CCl4 increased liver enzymes, UA, BU, and creatinine in the blood samples. Propolis significantly alleviated liver and kidney function, improved antioxidant parameters, and decreased levels of oxidative agents.
Conclusion: The data showed for the 1st time that Moroccan propolis has a protective effect against CCl4-induced kidney and liver toxicity by maintaining the activity of the antioxidant defense system, which was most likely due to its antioxidant activity. Keywords: antioxidant, carbon tetrachloride, kidney, liver, propolis, toxicity.
Keywords: antioxidant, carbon tetrachloride, kidney, liver, propolis, toxicity.
How to cite this article: El-Haskoury R, Al-Waili N, Kamoun Z, Makni M, Al-Waili A, Lyoussi B (2021) Antioxidant activity and protective effect of propolis against carbon tetrachloride-induced liver and kidney injury by modulation of oxidative parameters, Veterinary World, 14(12): 3076-3083.
Received: 30-05-2021 Accepted: 25-10-2021 Published online: 08-12-2021
Corresponding author: Noori Al-Waili E-mail: noori786@yahoo.com
DOI: 10.14202/vetworld.2021.3076-3083
Copyright: El-Haskoury, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.