Vet World   Vol.15   August-2022  Article-9

Changes in rumen fermentation and bacterial profiles after administering Lactiplantibacillus plantarum as a probiotic

Background and Aim: Lactiplantibacillus plantarum is one of the lactic acid bacteria that is often used as probiotics. This study aimed to evaluate the effects of Lactiplantibacillus plantarum TSD10 as a probiotic on rumen fermentation and microbial population in Ongole breed cattle.

Materials and Methods: This study adopted an experimental crossover design, using three-fistulated Ongole breed cattle. Treatments were as follows: T0, control without probiotic; T1, 10 mL probiotic/day; T2, 20 mL probiotic/day; and T3, 30 mL probiotic/day. The basal diet of the cattle comprised 70% concentrate: 30% elephant grass (Pennisetum purpureum). The concentration of probiotic used was 1.8 × 1010 colony-forming unit (CFU)/mL.

Results: We observed significantly lower acetate production compared with control (64.12%), the lowest values being in the T3 group (55.53%). Contrarily, propionate production significantly increased from 18.67% (control) to 23.32% (T2). All treatments yielded significantly lower acetate–propionate ratios than control (3.44), with the lowest ratio in the T3 group (2.41). The protozoal number decreased on probiotic supplementation, with the lowest population recorded in the T2 group (5.65 log cells/mL). The population of specific rumen bacteria was estimated using a quantitative polymerase chain reaction. We found that the population of L. plantarum, Ruminococcus flavefaciens, and Treponema bryantii, did not change significantly on probiotic supplementation, While that of Ruminococcus albus increased significantly from 9.88 log CFU/mL in controls to 12.62 log CFU/mL in the T2 group.

Conclusion: This study showed that the optimum dosage of L. plantarum TSD10 as a probiotic was 20 mL/day. The effect of L. plantarum as a probiotic on feed degradation in rumen was not evaluated in this experiment. Therefore, the effect of L. plantarum as a probiotic on feed degradation should be performed in further studies. Keywords: Lactiplantibacillus plantarum, microbes, probiotic, rumen.

Keywords: Lactiplantibacillus plantarum, microbes, probiotic, rumen.

How to cite this article: Astuti WD, Ridwan R, Fidriyanto R, Rohmatussolihat R, Sari NF, Sarwono KA, Fitri A, and Widyastuti Y (2022) Changes in rumen fermentation and bacterial profiles after administering Lactiplantibacillus plantarum as a probiotic, Veterinary World, 15(8): 1969–1974.

Received: 18-02-2022  Accepted: 10-06-2022     Published online: 19-08-2022

Corresponding author: Wulansih Dwi Astuti   E-mail: wulan_nie@yahoo.com

DOI: 10.14202/vetworld.2022.1969-1974

Copyright: Astuti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.