Vet World Vol.17 October-2024 Article - 12
Research Article
Veterinary World, 17(10): 2304-2310
https://doi.org/10.14202/vetworld.2024.2304-2310
Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows
2. PT Ultra Peternakan Bandung Selatan, Pangalengan, Kabupaten Bandung, Jawa Barat 40378, Indonesia.
3. Research Center for Food Technology and Processing, The Indonesian National Research and Innovation Agency, Gunung Kidul, Daerah Istimewa Yogyakarta 55861, Indonesia.
4. Research Center for Animal Husbandry, The Indonesian National Research and Innovation Agency, Bogor, Jawa Barat - 16911, Indonesia.
Background and Aim: Milk physicochemical properties play essential role in the milk processing industry, which are moderately to highly affected by genetic factors. This study aimed to evaluate the association between single nucleotide polymorphisms in POU class 1 homeobox 1 (POU1F1) and the physicochemical properties of milk in high-producing Holstein Friesian (HF) cows.
Materials and Methods: A total of 149 high-producing dairy cows from PT Ultra Peternakan Bandung Selatan was included in this study. The physicochemical properties of milk, including density, freezing point, pH, lactose, solid non-fat, protein, and ash content, were determined. Moreover, three polymorphisms within the exon regions of POU1F1 (c.195G>A, c.300G>T, and c.828G>A) were analyzed using polymerase chain reaction-restriction fragment length polymorphism. The association between these polymorphisms and the physicochemical properties of milk was determined using a mixed-effects model analysis, in which the lactation period was used as a covariate.
Results: This study found that two polymorphisms, c.195G>A and c.828G>A, significantly affected the pH of fresh milk. Cows with both the GG genotypes c.195G>A and c.828G>A had lower milk pH values than those with the other genotypes. In addition, a non-significant effect of POU1F1 was observed on the other physicochemical properties of milk.
Conclusion: Two POU1F1 polymorphisms determined the pH of fresh milk in the Indonesian HF population. These are potential marker candidates for milk pH that directly affect the development of dairy products in the milk processing industry.
Keywords: exonic mutation, Holstein Friesian, milk pH, physicochemical properties, POU1F1.
How to cite this article: Cahyadi M, Hasan AI, Sakti DS, Nissa NA, Pramono A, Firmanto S, Friyatna RN, Volkandari SD, and Sudrajad P (2024) Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows, Veterinary World, 17(10): 2304-2310.
Received: 2024-07-01 Accepted: 2024-09-10 Published online: 2024-10-17
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.2304-2310
Copyright: Cahyadi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
