Vet World Vol.18 February-2025 Article - 2
Research Article
Veterinary World, 18(2): 263-269
https://doi.org/10.14202/vetworld.2025.263-269
Green-synthesized zinc oxide nanoparticles from Garcinia mangostana leaf extract: A promising antibacterial agent for poultry
2. Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.
3. Research Center for Animal Husbandry, The National Research and Innovation Agency, Jakarta, 10340, Indonesia.
4. Study Program of Livestock Product Processing Technology, Politeknik Negeri Banyuwangi, Banyuwangi, 68461, Indonesia.
Background and Aim: The development of zinc-based antimicrobials has progressed from conventional to nanoparticle (NP) formulations due to their enhanced biological properties. This study evaluates the antibacterial efficacy of green-synthesized zinc oxide NPs (ZnO-NPs) derived from Garcinia mangostana leaf extract against both pathogenic and non-pathogenic bacteria relevant to poultry health.
Materials and Methods: Using a completely randomized design, six treatment groups were tested: Positive control (PC) (tetracycline, 2.5 mg/mL), negative control (NC) (HCl 0.1 N + distilled water), and ZnO-NPs at concentrations of 2.5, 5, 7.5, and 10 mg/mL. The antimicrobial activity was assessed using the agar well diffusion method, measuring inhibition zones against Escherichia coli, S. Typhimurium, Staphylococcus aureus, and Lactobacillus plantarum.
Results: The positive control demonstrated the most significant inhibition zones across all bacterial strains. ZnO-NPs exhibited dose-dependent antibacterial activity, with maximum inhibition zones recorded as 18.58 mm for E. coli, 17.09 mm for S. Typhimurium, and 17.41 mm for S. aureus at the highest concentration (10 mg/mL). However, the antibacterial activity against L. plantarum was less pronounced, with a maximum inhibition zone of 9.93 mm. These findings indicate selective toxicity of ZnO-NPs, favoring pathogenic over non-pathogenic strains.
Conclusion: Green-synthesized ZnO-NPs using G. mangostana leaf extract demonstrate promising antibacterial properties against poultry pathogens. Their selective action highlights potential applications as eco-friendly feed additives to improve poultry health and mitigate pathogenic risks.
Keywords: antibacterial, green synthesis, nanotechnology, poultry health, zinc oxide nanoparticles.
How to cite this article: Rusli RK, Zurmiati Z, Mutia R, Reski S, Darmawan A, Hidayat C, Mahata ME, Hilmi M, and Yuniza A (2025) Green-synthesized zinc oxide nanoparticles from Garcinia mangostana leaf extract: A promising antibacterial agent for poultry, Veterinary World, 18(2): 263-269.
Received: 2024-10-03 Accepted: 2024-12-31 Published online: 2025-02-08
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2025.263-269
Copyright: Rusli, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
