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Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle

The aim of the present study was to evaluate the milk amyloid A (MAA) in the milk of normal and SCM cows and compare the sensitivity of both MAA secretion and SCC in response to mammary gland bacterial infection

Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis

Bovine visceral schistosomiasis has been reported as an important disease entity as it affects animal health, productivity, causes economic losses due to liver condemnation, and produces a high morbidity

Development of indirect enzyme-linked immunosorbent assay for diagnosis of canine leptospirosis

This study was taken up to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for screening antibodies against Leptospira spp. in canines

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India

Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, nutrition, gynecology, surgery…

Association of milk microbiome with bovine mastitis before and after antibiotic therapy

This study aimed to use molecular methods to identify milk microbiome patterns characteristic of mastitis that can help in the early diagnosis of this disease and in the development of new treatment strategies.

Accuracy of molecular diagnostic methods for the detection of bovine brucellosis: A systematic review and meta-analysis

investigate the diagnostic accuracy of molecular tools, in comparison to gold standard bacterial isolation and serological assays for the diagnosis of bovine brucellosis

Bacteriological and molecular characterization of mastitis-associated pathogens and milk quality in ewes and goats in Al-Kut, Iraq

This study aimed to determine the occurrence of California Mastitis Test (CMT)-positive milk samples in ewes and goats, assess N-acetyl-β-D-glucosaminidase (NAGase) reaction intensity, characterize associated bacterial pathogens using bacteriological and mole…

Recombinant IpaJ protein for serological detection of Salmonella enterica serovar Pullorum infection in chickens: Production, characterization, and diagnostic evaluation

This study aimed to produce and characterize recombinant IpaJ (rIpaJ) and preliminarily evaluate its diagnostic performance using field-derived chicken sera from Kazakhstan, compared with a commercial LPS-based enzyme-linked immunosorbent assay (ELISA).

Quantitative polymerase chain reaction detection of hemotropic and vector-borne pathogens in dogs and cats using blood and pooled blood–urine matrixes: A large-scale retrospective study in Colombia

This study compared whole blood with a pooled BU matrix for quantitative polymerase chain reaction (qPCR) detection of hemotropic and vector-borne pathogens in dogs and cats in Colombia.

Molecular detection of latent feline leukemia virus infection using pol gene polymerase chain reaction in an enriched clinical cohort of Thai cats

This study aimed to evaluate a polymerase chain reaction (PCR) assay targeting the conserved FeLV pol gene for molecular detection of FeLV and to compare its diagnostic performance with a commercial p27 antigen rapid immunoassay in an enriched clinical cohort…

Epidemiology, frost resistance, and emerging species-specific diagnostics for Trichinella nativa: Current evidence and future priorities

This review summarizes current knowledge on the taxonomy, epidemiology, transmission, pathogenesis, clinical manifestations, and diagnostic approaches for T. nativa, with particular emphasis on recent advances in molecular and immunological detection methods.

Comparative evaluation of lightweight and pre-trained deep learning models for multi-class classification of infected freshwater fish species in Thailand

Comparative evaluation of lightweight and pre-trained deep learning models for multi-class classification of infected freshwater fish species in Thailand

Development and validation of a recombinant capsid protein-based indirect enzyme-linked immunosorbent assay for serological detection of duck circovirus in commercial flocks

This study aimed to develop and validate an indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant capsid (Cap) protein for sensitive and specific detection of antibodies against DuCV.

Comparative immunodiagnostic evaluation of recombinant serine proteases from Trichinella spiralis and Trichinella nativa for early detection of trichinellosis

This study aimed to clone, express, and evaluate recombinant serine proteases from Trichinella spiralis (rTsp-LE) to Trichinella nativa (rTnsp-4E), and to assess their diagnostic performance compared with ES antigens and a commercial kit.

Immunological insights into the occurrence of Lawsonia intracellularis in horses from southern Brazil using flow cytometry