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Development and validation of a quadruplex real-time quantitative polymerase chain reaction assay for the simultaneous detection of Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Actinobacillus pleuropneumoniae, and Glaesserella parasuis

This study aimed to develop and validate a quadruplex real-time quantitative polymerase chain reaction (qPCR) assay for the simultaneous detection of these four important porcine respiratory bacterial pathogens and evaluate its applicability using clinical sa…

Development and application of a quantitative polymerase chain reaction assay for the detection and genotyping of bovine leukemia virus in cattle from Kazakhstan

This study aimed to develop a sensitive and specific duplex quantitative polymerase chain reaction (qPCR) assay targeting the env gene of BLV with β-actin as an internal control and apply it for molecular surveillance and genotyping of BLV in cattle from six …

A comparison of diagnostic methods for canine Ehrlichiosis: Microscopy and RNases hybridization-assisted amplification technology compared with the quantitative polymerase chain reaction

This study aimed to determine the prevalence of major canine vector-borne pathogens in Bangkok, Thailand during the 2024 rainy season and evaluate the diagnostic performance of microscopy and a novel RNase hybridization-assisted amplification (RHAM) test kit …

Molecular detection, characterization, and cross-species circulation of feline and canine parvoviruses in Gujarat, India: Emergence of a novel canine parvovirus-2c variant and enhanced diagnostic sensitivity of quantitative polymerase chain reaction

This study aimed to determine the prevalence of FPV and CPV infections among cats and dogs in Gujarat, India, compare the diagnostic performance of rapid antigen testing (RAT), conventional polymerase chain reaction (PCR), and quantitative PCR (qPCR), charact…

Molecular surveillance confirms absence of avian influenza virus type A in Ecuadorian poultry: A retrospective reverse transcription quantitative polymerase chain reaction study from April 2023 to June 2024

This study aimed to confirm the absence of AI virus type A in poultry across six major provinces of Ecuador following the 2022 outbreak, as part of the national eradication and vaccination campaign.

Molecular detection and quantification of canine parvovirus 2 using a fast and sensitive SYBR® green-based quantitative polymerase chain reaction assay in dogs affected with gastroenteritis

Evaluation of early African swine fever virus detection using CP204L gene encoding the p30 protein using quantitative polymerase chain reaction

This study investigated the capability of detecting ASFV early through the analysis of genes B646L and CP204L, encoding .....

Quantitative polymerase chain reaction detection of hemotropic and vector-borne pathogens in dogs and cats using blood and pooled blood–urine matrixes: A large-scale retrospective study in Colombia

This study compared whole blood with a pooled BU matrix for quantitative polymerase chain reaction (qPCR) detection of hemotropic and vector-borne pathogens in dogs and cats in Colombia.

Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India

The study was undertaken to detect the pathogenic Leptospira in healthy dogs’ urinary shedding by real-time polymerase chain reaction (qPCR)

Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

Shiga toxin-producing Escherichia coli (STEC) and develop a quantitative polymerase chain reaction (qPCR) assay to quantify the bacterial DNA present in different food matrices

Presence of toxin-producing bacterial pathogens and associated risk factors in neonatal diarrhea of piglets on commercial sow farms in Vietnam

This study aimed to assess the prevalence of Escherichia coli, Clostridium perfringens, and Clostridioides difficile and to identify risk factors contributing to NPD on Vietnamese sow farms.

One-step triplex TaqMan quantitative reverse transcription polymerase chain reaction for the detection of feline coronavirus, feline panleukopenia virus, and feline leukemia virus

This study aimed to establish a reliable and rapid method for accurate detection of FCoV, FPV, and FeLV so that a definite diagnosis can be made and effective measures can be taken to prevent and control viral infection.

Stability of African swine fever virus genome under different environmental conditions

This study aimed to investigate the stability of ASFV under different environmental conditions from both genomic and antibody perspectives, and to provide a theoretical basis for the prevention and elimination of ASFV.

High prevalence of Coxiella burnetii infection in humans and livestock in Assiut, Egypt: A serological and molecular survey

The aim of this study was to identify the seroprevalence of C. burnetii in humans and livestock and to test for the presence of C. burnetii DNA in sera from seropositive animals and humans

Evaluation of a modified method of extraction, purification, and characterization of lipopolysaccharide (O antigen) from Salmonella Typhimurium

we evaluated its immunopotentiating function by measuring the relative RNA expression levels of splenic immune-related genes