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Selection of two pronuclei zygotes before electroporation does not improve gene editing efficiency or reduce mosaicism in porcine embryos despite optimized timing and Hoechst staining

This study investigated the effects of 2PN selection, optimized timing of pronuclear observation, and Hoechst 33342 staining conditions on embryo development and genome editing efficiency in porcine embryos.

Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos

The study aimed to determine the most effective concentration of L-carnitine to improve the oocyte developmental competence and cryotolerance of buffalo embryos

Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock.

Integrating morphological embryo quality scoring and untargeted metabolomics identifies candidate biomarkers in Bali cattle embryos

This study aimed to evaluate Bali cattle embryo quality and identify candidate metabolite biomarkers by integrating multi-parameter morphological scoring with liquid chromatography–high-resolution mass spectrometry (LC–HRMS)-based untargeted metabolomics.

Efficient gene editing of pig embryos by combining electroporation and lipofection

Fowl adenovirus 8a isolated from chickens with runting and stunting syndrome induces inclusion body hepatitis and hepatitis-hydropericardium syndrome in chicken embryos

Curcumin enhances developmental competence and ameliorates heat stress in in vitro buffalo (Bubalus bubalis) embryos

Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods

The combination of CRISPR/Cas9 delivery methods may improve the mutation efficiency of triple-gene edited porcine blastocysts

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection

This study aimed to determine that a combination of microinjection and EP of CRISPR/Cas9 system could increase the rates of biallelic mutation for triple-gene knockout in porcine blastocysts

The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method

Identify the deoxyribonucleic acid (DNA) profile and changes of post-warming embryo after being frozen with vitrification method using microsatellite method

The use of carotene-containing preparation in cows for the prevention of postpartum complications

The aim of this study was to assess the efficiency of using carotene-containing preparation in the prevention of postpartum complications in cows

Effect of exposure to different light colors on embryonic development and neurophysiological traits in the chick embryo

this study aimed to demonstrate the importance of light and its various colors on the growth and development of broiler chick embryos