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47 articles matching “polymerase chain reaction.”

17
Research Article25 Mar 2022Vol. 15 · Issue 3

Systematic evaluation of TaqMan real-time polymerase chain reaction assays targeting the dsb and gltA loci of Ehrlichia canis in recombinant plasmids and naturally infected dogs

Sarawan Kaewmongkol

This study evaluated the amplification efficiency, linearity, precision, and sensitivity of two TaqMan RT-PCR assays targeting the dsb and gltA loci of E. canis in recombinant plasmids and naturally infected dogs

Read article ↗6 citations
18
Research Article10 Nov 2021Vol. 14 · Issue 11

Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand

Sirikanda Thanasuwan

This study aimed to determine the distribution of Fasciola spp. and compare the specificity and sensitivity of FECT under microscopy to that of polymerase chain reaction (PCR) in cattle feces

Read article ↗4 citations
19
Research Article27 Aug 2021Vol. 14 · Issue 8

Diagnosis of naturally occurring lumpy skin disease virus infection in cattle using virological, molecular, and immunohistopathological assays

Rabab T. Hassanien

The present study was designed to compare the results of different diagnostic approaches used for the diagnosis of LSD virus (LSDV)

Read article ↗24 citations
20
Research Article07 Aug 2021Vol. 14 · Issue 8

Biological and molecular characterization of a sheep pathogen isolate of Mannheimia haemolytica and leukotoxin production kinetics

Dounia Bkiri

This study reports the biological and molecular characteristics of a new Moroccan Mha isolate from small ruminants presenting typical respiratory symptoms

Read article ↗4 citations
21
Research Article27 Feb 2021Vol. 14 · Issue 2

The occurrence of elephant endotheliotropic herpesvirus infection in wild and captive Asian elephants in Thailand: Investigation based on viral DNA and host antibody

Tuempong Wongtawan

we investigated the occurrence of EEHV and the efficiency of different techniques used to monitor EEHV infection in various samples and populations of Asian elephants

Read article ↗11 citations
22
Research Article19 Jan 2021Vol. 14 · Issue 1

Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera

Nour H. Abdel-Hamid

this study aimed to evaluate the diagnostic performance of the TaqMan Real- Time-PCR (RT-PCR) targeting the bcsp31 gene versus conventional PCR for the accurate diagnosis of brucellosis at the genus level in cattle sera

Read article ↗11 citations
23
Research Article05 Dec 2020Vol. 13 · Issue 12

High prevalence of Coxiella burnetii infection in humans and livestock in Assiut, Egypt: A serological and molecular survey

Salah Abdel Kareem Selim

The aim of this study was to identify the seroprevalence of C. burnetii in humans and livestock and to test for the presence of C. burnetii DNA in sera from seropositive animals and humans

Read article ↗23 citations
24
Research Article28 Oct 2020Vol. 13 · Issue 10

Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Azhar G. Shalaby

Flinders Technology Associates (FTA) cards simplify sample storage, transport, and extraction by reducing cost and time for diagnosis

Read article ↗6 citations
26
Research Article02 Sep 2020Vol. 13 · Issue 9

Listeriosis in a peri-urban area: Cultural and molecular characterization of Listeria monocytogenes isolated from encephalitic goats

Nagendra Nath Barman

This study reports the cultural, pathological, and molecular characterization of Listeria monocytogenes isolated from encephalitic goats from peri-urban Guwahati, Assam

Read article ↗6 citations
29
Research Article24 Mar 2020Vol. 13 · Issue 3

Molecular characterization of foot-and-mouth disease viruses collected from Northern and Central Ethiopia during the 2018 outbreak

Fazlurrahman Khan

This study aimed to isolate and characterize the FMDVs using reverse transcription-polymerase chain reaction (RT-PCR) and gene sequencing

Read article ↗14 citations
30
Research Article24 Jan 2020Vol. 13 · Issue 1

Multilocus analysis of Gallid herpesvirus 1 in layer chickens in Iraq

Mohammed Hamzah Abdulkadhim Al-Saadi

This study was performed to explore the multilocus variation of ILTV strains of field and vaccine origin

Read article ↗2 citations